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a perswasive essay I take my dog Oskar to work with me nearly every day. He rides in massage a trailer that I tow behind my bike 2.5 miles uphill to the Kibin office. I’m lucky that I work for a place that allows dogs. Other dog companions aren’t so fortunate. Essays? Shouldn’t responsible dog owners be allowed to bring their beloved furballs anywhere they wish? But, this post isn’t about massage research, teaching you to persuade your boss to let you bring your dog to work (although that would be cool). Mla Cover Research? No, the goal of this blog post is to teach you how to write a persuasive essay outline. In this post, I’ll break down the components of a good persuasive essay. I’ll also set you up with a downloadable outline template that you can use when you are ready to infant persuade your teacher to give you a better grade in English class this semester. What is a Persuasive Essay Anyway?

The goal of a persuasive essay is to convince your readers that your viewpoint is the hybrid car research paper, right viewpoint. Infant Research Papers? In a persuasive paper, you pull out all the stops to say, “It’s my way or the bad grammar essays, highway!” Unlike argumentative essays, where facts reign supreme, you don’t necessarily have to use researched, absolute facts to infant papers support your persuasive paper. The goal of your persuasive paper is to persuade by senior any means necessary. If that involves including emotional anecdotes or stories instead of facts, that’s fine. Massage? Don’t believe me? Ask any politician. When it comes to car research powers of persuasion, the facts don’t necessarily matter.

While including actual facts and evidence can be an research papers effective way to essay racism in austria persuade, it’s okay to play dirty in infant a persuasive essay. Bad Grammar? Make your readers laugh, cry, or quake in fear as long as it gets them to believe that what you are saying is true. That said, you can’t go in and write your essay without any direction. To really persuade someone in massage papers your persuasive essay, you have to essays zen buddhism be smoooooth . Infant Papers? You have to hybrid paper have finesse. To be smooth and finesseful (not a word, by the way), you should start with an outline. Infant Massage Research Papers? Here’s an essay example of infant massage papers, a persuasive essay outline: First, it’s important to hybrid select a topic that you can take a stand for. Infant Massage Papers? Let’s say we’re writing about animal rights. I’m not talking about essays, your typical “people shouldn’t hurt animals” essay.

I’m talking about infant papers, bestowing actual human rights on to my favorite animal: dogs. 1. Write a hook . I’ve said it before, and I’ll say it again. Always start your introduction with a strong hook. Make your audience want to read your essay. For example, “Your dog is smarter than your baby, and more useful and loyal too. Essays Zen Buddhism Suzuki? Plus, your dog will never, ever turn into an angsty teenager. If dogs are such good people, why don’t they have rights?” Define your audience . This is a sentence or two that helps your reader define himself as being a member of your target audience. In my example, I’m specifically speaking to dog owners who live in Portland, Oregon. For example, “For all Portland dog companions who have ever been turned away from a restaurant, disallowed access to transit, or rejected from a public park, it’s time to stand up for your pet’s rights—and your rights too!” 2. Massage Research? Present your thesis statement . Here is where you get to the meat of your persuasive essay and define the exact viewpoint that you want your audience to adopt. Much as you would in an argumentative essay, you must take a stance on your topic. No wishy-washy “eh, I could go either way” stuff allowed.

Pick a side. Mla Cover Page Research Paper? Stick to it. Defend it to the end! For example, “The dogs of Portland deserve the same rights and privileges as granted to our youngest humans, such as the right to visit restaurants, ride buses, walk off-leash, and go to the cinema so long as they are accompanied by a responsible human companion.” Persuasive Essay Outline Body Paragraphs. The exact number of body paragraphs you include will depend on the parameters of your assignment and infant massage research your topic.

A bigger assignment and/or topic will require more reasons and paragraphs. Zen Buddhism Suzuki? A smaller assignment and/or topic will require fewer reasons and paragraphs. For the purpose of infant papers, this blog post, I’m including three example reasons. Each reason you come up with can be emotionally charged, logically irrefutable, or ethically binding—so long as it’s persuasive. In addition, each persuasive reason you offer should be supported by a fact or an for college example. Reason #1. Portland dogs are as smart as young children and often make for more polite companions. Fact or example 1 : Dogs are capable of learning up to infant research 250 words and can easily go with the flow of human interactions. Fact or example 2 : Dogs are quieter and less disruptive than the comparaticve essay, average two-year-old human. Original photo by dharmabumx (Creative Commons) Reason #2. Massage? Portland dogs should be able to walk leash-free if they are accompanied by their human companions; in most cases, wearing a leash is unnecessary.

Fact or example 1 : Dogs can be easily trained to walk alongside their human companions without a leash or restraint. Fact or example 2 : In a recent survey, 65% of essay in austria, Portland dog owners said that walking a dog on a leash is more of a hassle than walking a dog leash-free. Reason #3 . More rights for Portland dogs means more rights for Portland’s dog companions. Fact or example 1 : Dog companions will have more choices of places where they can spend time with friends and family without having to leave beloved pets behind. Massage? Fact or example 2 : Dog companions won’t have to deal with the trouble of hiring a dog-walker while at work or a pet-sitter for short weekend getaways. Mla Cover Page Research Paper? Persuasive Essay Outline Conclusion. Now that you have outlined your reasons and supporting facts and examples, it’s time to infant seal the deal in your essay’s conclusion. Your conclusion should contain the following important components: 1. Brief summary . Remind your audience of why this topic is important.

For example, “Dogs all across Portland are being unfairly denied the basic right to accompany their human companions to public places. It’s time for Portland dog owners to stand up for senior essay their furry friends.” 2. Benefits to research papers the reader . Explain how acting on this issue will benefit your audience. For example, “Not only will taking a stand for your canine benefit dogs everywhere, it will also benefit you. Next time you want to bad grammar take a weekend away, ride the bus to work, or enjoy a matinee, you won’t have to worry about who will take care of your dog while you are away. This issue is about your rights as a dog companion too.” 3. A call to action . Infant Massage Research? What do you want your readers to do now that they’ve (hopefully) subscribed to your viewpoint on the topic? For example, “Vote ‘yes’ on Portland City Ballot initiative 14 this election. It’s time to stand up for bad grammar essays the rights of our most loyal friends.” Downloadable Persuasive Essay Outline Template and Additional Resources.

Now that you have a better idea of what it takes to create a persuasive essay outline, go forth and persuade the infant, world! I created the above visual outline using the online mind-mapping app at page research text2mindmap. It’s a great resource to brainstorm your persuasive essay topic, or create a visual persuasive essay outline. Here is another useful persuasive essay outline builder that I found during my research. Infant Massage Research? Feel free to use it to get started. Once your writing is complete, be sure to have an editor review your essay for you. After all, you don’t want all your preparation to be for essays nothing.

Psst. 98% of Kibin users report better grades! Get inspiration from over 500,000 example essays. About the papers, Author. Naomi Tepper is a former Kibin editor, the former content manager for senior for college the Kibin blog, and forever a word nerd. Hi Naomi, it was outstanding.

The way you used to describe the outline of an massage research papers essay was just fabulous. And the hybrid paper, #8220;PICK STICK DEFEND#8221; was also great. keep it up you are doing good work. For me atleast. REGARDS. Happy to help! Thanks for reading. #128578; Probably why you#8217;re failing English. nah actually got a A- Congratulations! 5th grade English was pretty easy for me too. Sweet, thanks for the comment #128578; I#8217;m a student from the massage research papers, Philippines. I#8217;ve got to commend you for doing a great job, it is an easy read that can be understood not just by college students.

I#8217;m a grade 10 student and this really helped me a lot for my upcoming project. Bad Grammar? Keep up the good work and infant papers May God Bless You! Thanks for reading, and I know that Naomi will appreciate the outline, kind words, Rebekka #128578; I#8217;m using this outline now. Looks useful. Sweet! Glad you find it helpful for your essay! Thanks for the comment.

I#8217;m in infant massage research grade 7 and this really helped with my essay. Thanks! Awesome, so glad you found it helpful! Thank you for reading #128578;

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Essay: Gene transfer techniques – pros and cons. Gene transfer is infant massage papers, a technique to stably and efficiently introduce functional genes (that are usually cloned) into the target cells. Genetic transfer is the mechanism by which DNA is transferred from a donor to a recipient. Page! Once donor DNA is inside the recipient, crossing over can occur. The result is a recombinant cell that has a genome different from either the donor or the recipient. A recombination event must occur after transfer in order that the change in the genome be heritable (passed on to the infant papers next generation).The genes are the blueprints essential to generate all the proteins in our bodies which eventually perform all the biological functions. Therefore, when a gene is efficiently introduced into a target cell of the host, the protein which is encoded by that gene is produced . Gene transfer technologies developed initially as a research tool for studying the gene expression and page paper, its function.

A range of techniques and infant massage research, naturally occurring processes are utilized for the gene transfer. Techniques are available for the introduction of DNA into many different cell types in culture, either to study gene function and regulation or to produce large amounts of zen buddhism suzuki recombinant protein. Cell cultures have therefore been used on infant research a commercial scale to synthesize products such as antibodies, hormones, growth factors, cytokines and viral coat proteins for immunization.The most important application of bad grammar essays this technology is in vivo gene therapy, i.e. the introduction of DNA into the cells of live animals in order to massage research papers, treat disease. The extraordinary potential of altered DNA molecule is to give rise to outline, new life-forms that are better adopted for survival. Change in base sequence of DNA leads to change in protein causing disease condition which can be corrected by manipulation of papers gene. Opinion Racism! Much emphasis has been given to the gene manipulation in cardiovascular diseases, parkinson’s disease, lysosomal disorders, ocular gene therapy and osteoarthritis. Overview of gene-transfer strategies: Gene transfer can be achieved essentially via three routes. The most straightforward is direct DNA transfer, the physical introduction of foreign DNA directly into the cell.

For example, in cultured cells this can be done by microinjection, whereas for cells in vivo direct transfer is often achieved by bombardment with tiny DNA-coated metal particles. The second route is termed transfection, and this encompasses a number of techniques, some chemical and some physical, which can be used to persuade cells to take up DNA from their surroundings. The third is to package the infant massage DNA inside an. animal virus, since viruses have evolved mechanisms to naturally infect cells and introduce their own nucleic acid. The transfer of foreign DNA into page research paper, a cell by this route is termed transduction. The choice of methods of DNA transfer depends upon the target cells in which transformation will be performed.

It also depends upon the objectives of gene manipulation. The transfection may be either stable or transient. Although, choice of DNA transfer method is very important, the other important steps are selection of gene, isolation of gene, preparing recombinant DNA and selection of transformed cells. The regeneration of organism with new characteristics is also equally important. The delivery of genes in vitro can be done by infant research, treating the cells with viruses such as retrovirus or adenovirus, calcium phosphate, liposomes, particle bombardment, fine needle naked DNA injection, electroporation or any combination of these methods. These are the powerful tools for research and have possible applications in gene therapy. FACTORS TO CONSIDER WHEN SELECTING A GENE DELIVERY METHOD. The advancement of new technologies has made gene delivery both more efficient yet more confusing given the formidable array of reagents and methods now available. Determining the best gene delivery approach for any particular experiment requires careful consideration of a number of different factors as stated below. 1. Cellular Context or Environment – The context or environment of the cells (i.e., in vivo vs. Comparaticve Essay! in.

vitro vs. Research! ex vivo) to which nucleic acids will be delivered is the first factor to consider when. deciding upon a gene transfer method. The obstacles and challenges posed by delivery to in. vitro cell cultures are very different than those posed by in vivo or ex vivo cells, and require. different tools and techniques for overcoming them. For example, targeting nucleic acids to. particular cells while avoiding other cells is usually a major concern for in vivo gene transfer.

applications, sometimes a concern for ex vivo applications, but usually not a concern for bad grammar, in vitro. Another important issue that must be addressed when planning in vivo (but not in vitro) gene. delivery studies is the immunogenicity of the gene transfer system. This is particularly important when considering the use of certain viral vectors discussed previously, which may be highly immunogenic. Also related to immunogenicity is the issue of general safety, both for the experimenter and for the subject of any study utilizing transfection. Infant Massage Papers! Almost always, the use of viral vectors requires very extensive safety precautions than does the use of synthetic transfection methods. 2. Cell or Tissue Type – The origin of the cells or tissue to comparaticve, which nucleic acids will be.

transferred is typically the massage research second parameter considered when selecting a gene delivery. method. Car Research Paper! For a number of different reasons, the difficulty of cellular nucleic acid delivery varies. from cell type to cell type. Because of this, it is massage papers, always important to review the literature to. determine if the cell type of interest has previously been utilized for gene transfer experiments, which exact methods have been used, and what delivery efficiencies were achieved, i.e., what. percentages of cells actually transfected or transduced, with each method.

Certain cell types are often described as ‘notoriously difficult to transfect’. These include primary cell cultures of many varieties. Successful gene transfer to such cells. usually requires a good deal of research before selecting any particular gene delivery method. 3. Delivery Efficiency – For most applications, transfecting or transducing as many cells as.

possible is preferred, especially when it is necessary to page, detect the presence of rare transgene. products. Thus, it is massage research papers, important to determine the level of mla cover research paper delivery efficiency required for a given. application before deciding on a particular gene transfer method. Massage! In most cases, it is found that. viral vectors provide the highest delivery efficiencies. For some commonly used and easily transfected cell lines, cationic lipids nearly match viral vectors in their gene transfer efficiency, while providing safer and much quicker procedures.

4. Stable vs. Transient Gene Delivery – Gene transfer procedures are frequently categorized. by whether the delivered gene remains separate from the host cell chromosome or whether it is integrated into bad grammar essays, the host cell chromosome. In the first case, known as transient gene delivery, expression of the transgene typically dissipates after a given period of time – usually within. several days – because the expression vector is either degraded or expelled from the host cell. In the second case, known as stable gene delivery, expression of the transferred gene is.

prolonged, or stable, because the vector is integrated into the host cell chromosome. Obviously, different applications require different time periods of transgene expression. Thus, a careful. comparison between different gene delivery methodologies will allow a suitable choice for. generating the desired transient or stable expression.

In the case of viral vectors, determining whether a particular virus is suited for stable or transient. transduction is simply a matter of learning the general characteristics of the infant massage vector. In the case. of chemical or physical/mechanical gene transfer, determining whether a given method is. suitable for transient vs. stable delivery may be as easy as reading sales literature from the. manufacturer or checking primary research literature.

5. Type of essays Delivered Molecule – Another factor to consider when selecting a gene delivery. method is papers, what type of nucleic acid or other molecule is being transfected or transduced. For. example, a reagent that efficiently delivers plasmids may not efficiently deliver DNA. oligonucleotides. It is frequently easiest to simply review the essays zen buddhism suzuki literature to learn what methods have been used successfully, and to contact other researchers who have experience delivering the molecule of massage papers interest. 6. Hybrid! Cytotoxicity – Another issue that comes up frequently when choosing among different gene. delivery methods is the cytotoxicity of the infant research various methods and reagents. Paper! Some methods, such.

as electroporation and infant, gene guns, can be extremely harsh on cells and often result in the death. of a majority of cells. Also, the DEAE-dextran can be quite harsh on some cell types, especially. when a DMSO or glycerol shock step is used as part of the procedure. In some applications, such as when a small number of transfectants are required from a recalcitrant cell type, this is.

not a problem. In the majority of applications however, a high percentage of cell death is simply. not acceptable. The plethora of essays available cationic lipids and polymer reagents have varying. degrees of toxicity with different cell types, and their concentrations during transfection can be. optimized as needed to minimize cell death. 7. Suspension vs. Adherent cells – Most commonly transfected cells grow – and are. transfected – while attached to some type of support, whether that support is the tissue from. which they are derived, some inert membrane or scaffolding, or the surface of a tissue culture. plate.

Less common are cells that grow while suspended in massage medium, such as those derived from. blood and other bodily fluids. Suspension cells are generally more difficult to transfect; therefore, it may be necessary to paper, rely on massage papers harsher gene delivery methods, such as. electroporation, gene guns, microinjection, or viral vectors for acceptable transfection levels. Also, some cationic lipids can effectively transfect suspension cells, and should be tried when. possible due to mla cover research, their simplicity and cost-effectiveness. 8. Expertise – The degree of difficulty in performing the various transfection techniques can. vary significantly, and therefore the expertise and experience of the researcher is usually an. important factor to consider. For example, methods like microinjection viral transfection methods are invariably more complex than cationic lipids and polymer reagents. Also, of the chemical methods, calcium phosphate co-precipitation can be quite tricky due to its sensitivity to slight changes in experimental conditions.

9. Time – Another important factor to consider is the infant massage papers time required for gene delivery. Viral. methods, while highly efficient, can be quite time consuming, typically taking anywhere from page research, two weeks to one month to complete. In contrast, chemical and infant massage papers, physical/mechanical methods can usually be completed within a few days, but frequently provide lower transfection efficiencies. Thus, it is not uncommon to comparaticve essay outline, have to weigh the benefits of faster results and less labor against the benefit of higher efficiency. 10 Cost – Finally, cost can be a major factor when deciding on the best gene delivery.

method for a given application. The most expensive methods tend to infant research, be the. physical/mechanical methods, such as electroporation and gene guns, due to the specialized. equipment they require.Viral gene delivery is perhaps the second most expensive method, since it requires the vector reagents, extensive time and labor to complete lengthy vector preparation procedures, as well as proper disposal of viral waste materials. The third most expensive gene delivery methods utilize polymers and cationic lipids, which do not require any specialized equipment or lengthy viral vector preparation steps. Such reagents typically cost in outline the hundreds of infant research papers dollars, depending on the number of transfections to be completed.

Finally, the least expensive methods utilize calcium phosphate, which is a cheap and abundant mineral, and naked DNA, which obviously requires no transfection reagent cost, but can be highly inefficient. GENE TRANSFER TECHNIQUES. Transformation is the naturally occurring process of gene transfer which involves absorption of the genetic material by a cell through cell membrane causing the fusion of the foreign DNA with the native DNA resulting in the genetic expression of the received DNA. Transformation involves the uptake of “naked” DNA (DNA not incorporated into essay racism, structures such as chromosomes) by competent bacterial cells. Massage! Cells are only bad grammar, competent (capable of taking up DNA) at a certain stage of their life cycle, apparently prior to the completion of cell wall synthesis . Transformation is massage research, usually a natural method of gene transfer but as a result of bad grammar essays technological advancement originated the artificial or induced transformation. Massage Research! Thus there are two types called as natural transformation and artificial or induced transformation. Bad Grammar! In natural transformation, the foreign DNA attaches itself to infant massage, the host cell DNA receptor and with the help of the protein DNA translocase it enters the host cell. The presence of suzuki nucleases restricts the entry of two strands of the DNA, destroys a single strand thus allowing only one strand to enter the host cell. Massage! Any DNA that is not integrated into the chromosome will be degraded. This single stranded DNA mingles with the host genetic material successfully.

The artificial or induced method of transformation is essays, done under laboratory condition which is either a chemical mediated gene transfer or done by infant research papers, electroporation.. Genetic engineers are able to induce competency by putting cells in car research certain solutions, typically containing calcium salts. At the entry site, endonucleases cut the DNA into infant, fragments of 7,000-10,000 nucleotides, and the double-stranded DNA separates into single strands. The single-stranded DNA may recombine with the host’s chromosome once inside the cell. This recombination replaces the essays gene in the host with a variant – albeit homologous – gene. DNA from a closely related genus may be acquired but, in general, DNA is not exchanged between distantly related microbes. Not all bacteria can become competent. Conjugation is a means of gene transfer in many species of bacteria.

Cell-to-cell contact by a specialized appendage, known as the massage research F-pilus (or sex pilus), allows a copy of an F- plasmid (fertility plasmid) to transfer to a cell that does not contain the plasmid. On rare occasions an F-plasmid may become integrated in the chromosome of its bacterial host, generating what is known as an Hfr (high frequency of recombination) cell. Such a cell can also direct the synthesis of a sex pilus. As the chromosome of the Hfr cell replicates it may begin to cross the pilus so that plasmid and chromosomal DNA transfers to the recipient cell. Such DNA may recombine with that of its new host, introducing new gene variants. Plasmids encoding genes for virulence factors antibiotic-resistance are passed throughout populations of bacteria, and between multiple species of bacteria by conjugation. In the laboratory, conjugation can be used to transfer disrupted genes on a self-transmissible plasmid, to develop a mutant strain. A gene of interest from a recipient E. coli strain is opinion essay racism, cloned into massage, a self-transmissible vector and maintained in essays a donor strain for genetic manipulation. The deleted gene construct is then transferred by conjugation from the donor strain back into the recipient strain. Transduction is another method for papers, transferring genes from one bacterium to another; the transfer is mediated by bacteriophages. A bacteriophage infection starts when the virus injects its DNA into a bacterial cell.

The bacteriophage DNA may then direct the synthesis of new viral components assembled in bad grammar essays the bacterium. Bacteriophage DNA is replicated and infant massage research, then packaged within the phage particles. Early in the infective cycle the phage encodes an enzyme that degrades the DNA of the host cell. Some of these fragments of bacterial DNA are packaged within the bacteriophage particles, taking the place of phage DNA. As the number of phages increases it breaks open (lyse) the cell. When released from the infected cell, a phage that contains bacterial genes can continue to infect a new bacterial cell, transferring the bacterial genes. Sometimes genes transferred in this manner become integrated into the genome of their new bacterial host by homologous recombination. Page Paper! Such transduced bacteria are not lysed because they do not contain adequate phage DNA for viral synthesis and these phages can enter an infant research papers alternate life cycle called lysogeny. In this cycle, all the virus’s DNA becomes integrated into the genome of the host bacterium. The integrated phage, called a prophage, can confer new properties to the bacterium. Researchers use transduction as a means of gene transfer as it requires a media like virus for transferring genes from one cell to the other.

Viruses are thus as a tool to introduce foreign DNA from the selected species to the target organism. There are two types of transduction called as generalized transduction in essay in austria which any of the bacterial gene is transferred via the bacteriophage to the other bacteria and infant massage papers, specialized transduction involves transfer of limited or selected set of genes. Viral vectors for gene transfer: The use of viruses as vectors for senior, transduction, i.e. the introduction of genes into animal cells by exploiting the natural ability of the virus particle, within which the transgene is papers, packaged, to adsorb to the surface of the cell and gain entry is being employed . Due to the efficiency with which viruses can deliver their nucleic acid into cells and the high levels of replication. and gene expression it is possible to achieve, viruses have been used as vectors not only for gene expression in cultured cells but also for gene transfer to living animals.

Four classes of viral vector have been developed for use in human gene therapy and have reached phase 1 clinical trials. These are the retrovirus, adenovirus, herpesvirus and adenoassociated virus (AAV) vectors . Bad Grammar! Before introducing the individual vector systems,we discuss some general properties of viral transduction vectors. Transgenes may be incorporated into. viral vectors either by addition to the whole genome or by replacing one or more viral genes. This is generally achieved either by massage research, ligation (many viruses have been modified to comparaticve outline, incorporate unique restriction sites) or homologous recombination. For many applications, it is favourable to use vectors from which all viral coding sequences have been deleted.These amplicons (also described as ‘gutless vectors’) contain just the cis-acting elements required for packaging and infant research, genome replication. The advantage of such vectors is their high capacity for foreign DNA and the fact that, since no viral gene products are made, the vector has no intrinsic cytotoxic effects. Essays Suzuki! The choice of vector depends on the particular properties of the virus and the intended host, whether transient or stable expression is required and infant massage papers, how much DNA needs to be packaged. For example, icosahedral viruses such as adenoviruses and retroviruses package their genomes into bad grammar essays, preformed capsids, whose volume defines the maximum amount of foreign DNA that can be accommodated. Conversely, rod-shaped viruses such as the baculoviruses form the capsid around the massage genome, so there are no such size constraints.

There is no ideal virus for gene transfer ‘ each has its own advantages and disadvantages. In recent years, a number of bad grammar hybrid viral vectors have been developed incorporating the infant research papers beneficial features of two or more viruses. 1) viral vectors that especially efficient at transducing the intended cell type but not other cell types. 2) retroviruses are particularly proficient at delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 1) cannot deliver genes to terminally differentiated cells. 2) viral vectors may be highly immunogenic. TRANSFECTION BY CHEMICAL METHODS. One of the methods of gene transfer where the genetic material is research paper, deliberately introduced into the animal cell in view of studying various functions of proteins and the gene is transfection.

This mode of gene transfer involves creation of research pores on the cell membrane enabling the cell to receive the foreign genetic material. When transformation is comparaticve essay, carried out in eukaryotic cells it is termed as transfection. 1) Calcium phosphate mediated DNA transfer. The process involves a mixture of isolated DNA (10-100ug) with solution of. calcium chloride and potassium phosphate. Massage Research! . Essay For College! When the two are combined, a fine precipitate of the papers positively charged calcium and the negatively charged phosphate will form, binding the DNA to page paper, be transfected on its surface .Cells are then incubated with precipitated DNA either in solution or in massage papers tissue culture dish. A fraction of cells will take up the calcium phosphate DNA precipitate by a process not entirely understood but thought to be endocytosis. Transfection efficiencies using calcium phosphate can be quite low, in the range of 1-2 % . It can. be increased if very high purity DNA is used and the precipitate allowed to form slowly. 1. Frequency is very low.

2. Research! Integrated genes undergo substantial modification. 3. Many cells do not like having the solid precipitate adhering to them. and the surface of their culture vessel. 4. Massage Papers! Integration with host cell chromosome is random. 5, Due to bad grammar essays, above limitations transfection applied to somatic gene therapy.

This technique is used for introducing DNA into mammalian cells. This process has been a preferred method of infant identifying many oncogenes. 2)DNA transfer by DAE-Dextran method: DNA can be transferred with the help of DAE Dextran also. DAE-Dextran may be used in the. transfection medium in which DNA is senior for college, present. This is infant research papers, a polycationic, high molecular weight substance and bad grammar, is convenient for transient assays. The negatively charged DNA binds to infant, the polycation and the complex is bad grammar essays, taken up by infant, the cell via endocytosis.

If DEAE (diethylaminoethyl )-Dextran treatment is coupled with Dimethyl Sulphoxide (DMSO) shock, then upto 80% transformed cell can express the transferred gene. The advantage of this method is that, it is cheap, simple and can be used for transient cells. which cannot survive even short exposure of calcium phosphate. The major advantages of the technique are its relative simplicity and speed, limited expense, and remarkably reproducible interexperimental and intraexperimental transfection efficiency. Disadvantages include inhibition of essays zen buddhism cell growth and induction of heterogeneous morphological changes in infant massage research papers cells. Furthermore, the concentration of serum in the culture medium must be transiently reduced during the transfection. It does not appear to be efficient for the production of stable transfectants. For cells that grow in suspension, electroporation or lipofection is usually preferred, although DEAE-dextran-mediated transfection can be used. Transfer of DNA by polycation-DMSO: As calcium phosphate method of DNA transfer is reproducible and efficient but there is opinion racism, narrow range of optimum conditions.

DNA transfer by. Another polycationic chemical,the detergent Polybrene is carried out to increase the infant papers adsorption of DNA to the cell surface followed by for college, a brief treatment with 25-30% DMSO to increase membrane permeability and enhance uptake of DNA. In this method no carrier DNA is required and stable transformants are produced. This method works with mouse fibroblast and chick embryo. LIPOSOME MEDIATED GENE TRANSFER. Liposomes are spheres of lipids which can be used to infant research, transport molecules into the cells. These are artificial vesicles that can act as delivery agents for exogenous materials including transgenes.

They are considered as spheres of lipid bilayers surrounding the essay molecule to be transported and promote transport after fusing with the papers cell membrane. Liposomes for use as gene transfer vehicles are prepared by adding an appropriate mix of essays zen buddhism bilayer constituents to an aqueous solution of DNA molecules. In this aqueous environment, phospholipid hydrophilic heads associate with water while hydrophobic tails self-associate to exclude water from within the lipid bilayer. This self-organizing process creates discrete spheres of continuous lipid bilayer membrane enveloping a small quantity of DNA solution. The liposomes are then ready to be added to target cells. Germline transgenesis is possible with liposome mediated gene transfer.Lipofection is the most common and massage research, generally utilized gene transfer technique in essays the recent years and it utilizes cationic lipids. The combined DNA and cationic lipids act instantaneously to form structures called as lipoplexes that are more complex in research structure than the senior for college simple liposomes. Cationic lipids have a positive charge and infant massage research papers, these form cationic liposomes which interact with the negatively charged cell membrane more readily than uncharged liposomes. This leads to hybrid, a fusion between cationic liposome and the cell surface resulting in quick entry by endocytosis and the delivery of the DNA directly across the plasma membrane.

Cationic liposomes can be produced from a number of cationic lipids that are commercially available and sold as an in vitro-transfecting agent, termed lipofectin. However this pathway would usually result in the fusion of lipoplexes with lysosomes and undergo degradation. This problem is overcome by utilizing the massage neutral helper lipids, which are generally included along with the cationic lipid. Comparaticve Essay Outline! This allows entrapped DNA to escape the endosomes, reach the nucleus and get access to the cell’s transcriptional machinery.The endosomal structure is destroyed by increasing the osmotic pressure created by the lipids’ buffering action within the endosomes and by the fusion of the lipid with the endosomal membrane. The ability of a lipid to destroy endosomes is one of the main characteristics of a transfection reagent. 2. Long term stability.

4. Protection of nucleic acid from degradation. 5. particularly proficient at delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 6. cationic lipids effectively transfect suspension cells. 1) Cannot deliver genes to terminally differentiated cells. A rapid and simple technique for introducing genes into a wide variety of microbial, plant and animal cells, including E. coli, is electroporation. Electroporation uses electrical pulse to research papers, produce transient pores in essay outline the plasma membrane thereby allowing macromolecules into massage, the cells. These pores are known as electropores which allow the molecules, ions and water to hybrid, pass from one side of the infant research membrane to another. The electropores reseal spontaneously and the cell can recover. The pores can be recovered only page research, if a suitable electric pulse is applied .The formation of electropores depends upon the cells that are used and the amplitude and duration of the electric pulse that is applied to them. When subjected to infant massage, electric shock, cells take up exogenous DNA from the suspending solution. Mla Cover Page Paper! Once inside the cell, the DNA is integrated and the foreign gene will express.

A proportion of infant massage these cells become stably transformed and can be selected if a suitable marker gene is carried on the transforming DNA. The most critical parameters are the intensity and duration of the electric pulse, and these must be determined empirically for different cell types. Electric currents can lead to dramatic heating of the cells that can results in senior essay cell death. Heating effects are minimized by using relatively high amplitude, a short duration pulse or by using two very short duration pulses. However, once optimal electroporation parameters have been established, the method is simple to carry out and highly reproducible. Many different factors affect the efficiency of electroporation, including temperature, various electric-field parameters (voltage, resistance and research papers, capacitance), topological form of the DNA, and various host-cell factors (genetic background, growth conditions) and post-pulse treatment. General applications of electroporation. 1. Introduction of essays suzuki exogeneous DNA into papers, animal cell lines, plant protoplast, yeast protoplast and bacterial protoplast. 2. Electroporation can be used to increase efficiency of transformation or transfection of suzuki bacterial cells. 3. Wheat, rice, maize, tobacco have been stably transformed with frequency upto 1% by this method.

4. Genes encoding selectable marker may be used to introduce genes using electroporation. 5. Infant Research Papers! To study the transient expression of molecular constructs. 6. Essays Zen Buddhism! Electroporation of early embryo may result in the production of transgenic animals. 7. Hepatocytes, epidermal cells, haematopoietic stem cells, fibroblast, mouse T and B lymphocytes can be transformed by infant massage research, this technique. 8. Naked DNA may be used for gene therapy by applying electroporation device on animal cells. Advantages of electroporation. 1. Essays Zen Buddhism! Method is fast. 3. Applied for a number of cell types. 4. Simultaneously a large number of cell can be treated. 5. High percentage of stable transformants can be produced.

1)Limited effective range of. 1 cm between the electrodes. 2)Surgical procedure is required to place the electrodes deep into the internal organs. 3)High voltage applied to tissues can result in irreversible tissue damage as a result of massage thermal heating. The technique has high input costs, because a specialized capacitor discharge machine is required that can accurately control pulse length and amplitude.

4) Larger numbers of cells may be required than for other methods because, in hybrid many cases, the most efficient electroporation occurs when there is up to 50% cell death. Sonication is the act of applying sound energy to agitate particles in infant massage research papers a sample. Ultrasonic frequencies (20 kHz) are usually used, leading to paper, the process also being known as ultrasonication . Sonoporation, or cellular sonication, is the use of these ultrasonic frequencies for modifying the research permeability of the cell plasma membrane. This technique is usually used in molecular biology and senior essay, non-viral gene therapy in order to allow uptake of large molecules such as DNA into the cell, in a cell disruption process called transfection or transformation. Sonoporation employs the infant research papers acoustic cavitation of microbubbles to enhance delivery of such large DNA molecules. Although the essay early results could achieve only 10- to infant, 30-fold increases in transfection efficiency in vitro, technical refinements have lead to page research paper, enhancements of up to several thousand fold in vitro ,sufficient to encourage ultrasonication mediated gene transfer in vivo. 1) It is non-invasive and well tolerated, 2)extraordinary safety record over a wide range of frequency and intensity. 3)high levels of public acceptability and understanding.

there are highly sophisticated, flexible, cost-effective and research papers, readily available diagnostic and senior essay for college, therapeutic systems that can achieve site-specific transfer of ultrasound energy almost anywhere in the body, except perhaps the lung. 4) The bioactivity of this technique is similar to, and in some cases found superior to, electroporation. 5) in vivo is relatively nontoxic. 1)Extended exposure to low-frequency (MHz) ultrasound has been demonstrated to result in complete cellular death (rupturing) as well as microvascular hemorrhage and disruption of tissue structure, thus cellular viability must also be accounted for research papers, when employing this technique. 2) Whether ultrasound affects later steps in the transfection process, particularly plasmid entry into the nucleus, remains unclear. Sonoporation is under active study for the introduction of foreign genes in tissue culture cells, especially mammalian cells.

Sonoporation is also being studied for use in targetedGene therapy in vivo, in a medical treatment scenario whereby a patient is given modified DNA, and an ultrasonic transducer might target this modified DNA into specific regions of the patient’s body. In microinjection DNA can be introduced into essays zen buddhism, cells or protoplast with the massage research help of very fine needles or. glass micropipettes having the diameter of 0.5 to 10 micrometer. Opinion! The DNA may be directly delivered into the nucleus or in the cytoplasm. Some of the infant massage papers DNA injected may be taken up by senior essay, the nucleus. The desired gene in the form of infant massage plasmid or alone is injected directly into opinion essay, the plant protoplast. Injection into the nucleus is more efficient than that into the cytoplasm. Massage! Micro-injection is carried out with automatic equipments (robotics) using a micro-needle. Hybrid Car Research! Computerized control of infant massage research holding pipette, needle, microscope stage and video technology has improved the efficiency of this technique.

Advantages of microinjection. 1. Frequency of stable integration of DNA is far better as compare to other methods. 2. Hybrid! Method is infant massage papers, effective in transforming primary cells as well as cells in bad grammar established cultures. 3. Infant! The DNA injected in this process is essay outline, subjected to less extensive modifications. 4. Infant Papers! Mere precise integration of senior for college recombinant gene in limited copy number can be obtained.

2. Skilled personal required. 3.Has not been successful for many plant cells . 4. Embryonic cells preferred for manipulation. 5. Massage! Knowledge of mating timing, oocyte recovery is essential. 6. Method is useful for protoplasts and not for research paper, the walled cells. 7. Massage Research! Process causes random integration. 8. Rearrangement or deletion of host DNA adjacent to site of integration are common.

9. The technique is laborious, technically difficult, and limited to the number of cells actually injected. Applications of microinjection. 1. Essay! Process is applicable for plant cell as well as animal cell but more common for animal cells. 2. Technique is ideally useful for producing transgenic animal quickly. 3. Procedure is important for gene transfer to infant papers, embryonic cells. 4. Applied to inject DNA into plant nuclei. 5. Method has been successfully used with cells and protoplast of. tobacco, alfalfa etc. Fig: microinjection in mouse egg. Biolistics or particle bombardment is a physical method that uses accelerated microprojectiles to.

deliver DNA or other molecules into intact tissues and cells. Biolistics transformation is relatively new and novel method amongst the physical methods for artificial transfer of exogenous DNA. The nucleic acid is senior essay for college, delivered through membrane penetration at a high velocity, usually connected to microprojectiles like microscopic particles of tungsten or gold coated into cells using an electrostatic pulse, air pressure, or gunpowder percussion. As the infant research papers particles pass through the opinion essay in austria cell, the DNA becomes free to integrate into the plant-cell genome. The gene gun is a device that literally fires DNA into target cells . The DNA to papers, be transformed. into the bad grammar essays cells is coated onto microscopic beads made of either gold or tungsten. Beads are carefully. coated with DNA.

The coated beads are then attached to the end of the plastic bullet and loaded into. the firing chamber of the gene gun. An explosive force fires the bullet down the infant papers barrel of the gun. towards the target cells that lie just beyond the end of the barrel. When the bullet reaches the end of. the barrel it is caught and stopped, but the DNA coated beads continue on toward the senior essay target cells. Some of the beads pass through the cell wall into the cytoplasm of the target cells.

Here the bead and. the DNA dissociate and the cells become transformed. Once inside the target cells, the DNA is. solubilised and massage research papers, may be expressed. 1. Biolistics technique has been used successfully to transform soyabean, cotton, spruce, sugarcane, papaya, corn, sunflower, rice, maize, wheat, 2. Genomes of subcellular organelles have been accessible to genetic. manipulation by biolistic method. 3. Mitochondria of plants and chloroplast of chlamydomonas have been. 4. Method can be applied to filamentous fungi and yeast (mitochondria).

5. The particle gun has also been used with pollen, early stage. embryoids, meristems somatic embryos, plant cells, root section, seeds and pollen. 6. This approach has been shown to be effective for transferring transgenes into bad grammar, mammalian cells in vivo . 1. Requirement of protoplast can be avoided. Unlike electroporation and microinjection, this technique does not require protoplasts or even single-cell isolations and is applicable to even intact plant tissue. 2. Walled intact cells can be penetrated. 3. Massage! Manipulation of genome of subcellular organelles can be achieved. 4. This is research paper, also a highly mechanized or robotic mediated technique in which the. speed of the micro-projectile particles is controlled by foolproof mechanisms.

5) It is also gaining in use as a method for transferring DNA construct into whole animals. 6) This technique is research, fast, simple and essay racism, safe, and it can transfer genes to a wide variety of tissues. 7)there appears to be no limits to the size or number of genes that can be delivered. 1. Integration is random. 2. Requirement of infant research equipments. 3.It can be a challenge to page, obtain a sufficient number of cells modified by this method to see a biologically significant effect. A new technique is papers, presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an essays zen buddhism suzuki uv pulsed laser. A frequency-multiplied Laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions.

At the site of the beam impact, due probably to local temperature changes, the cell membrane modifies its permeability. As a consequence of the hit, circular areas, whose radius may be apparently regulated by changing the irradiation time and/or the radiation intensity (energy), appear on the wall, last for a short time and massage research papers, fade spontaneously . It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in opinion essay racism the medium, to slip into the cell. It is infant massage research, well established that a strong pressure wave, known as a laser-induced stress wave (LISW), accompanies laser-induced plasma. We have extended their method to deliver macromolecules, such as genes. Plasmid DNA (circular DNA residing in hybrid bacterial cytoplasm) is used as a vector of the gene of interest. We inject the massage papers plasmid into target tissue, on essays which a laser target is placed, and subject the target to irradiation with a high-intensity, nanosecond laser pulse to induce plasma and infant massage papers, hence an LISW. Senior! By placing optically transparent material on the target, the plasma is confined, resulting in an increase in the LISW’s impulse.

Interaction of tissue cells with the LISW allows plasmid to enter the cytoplasm. 1) it only takes advantage of the presence of phenol-red, a normal cell medium component, with no need of addition of extraneous substances; (2) it is a very mild treatment virtually suitable for any cell type and. (3) it allows transfection of selected cells even in the presence of cells of different type (providing that they are morphologically distinguishable). 4) . it is infant research papers, rapidly replacing virus mediated techniques as they have serious side effects caused by car research, immune response and research papers, limited targeting characteristics rendering them inappropriate for clinical application. 5) The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude. 6) . It enables minimally invasive tissue interaction. Thus there are a number of ways by which the genes can be introduced into the cells. With the advent of molecular tools and technologies it is hybrid car research, now comparatively easy to research, introduce gene into cells without losing its integrity and biological activity. Moreover the recent development in molecular biology has made the bad grammar essays transfer of gene with great accuracy to the target cell. The transfer of gene through different gene transfer technologies has cured a number of diseases. Massage! Research is on progress to cure those diseases which cannot be cured by opinion essay in austria, using drugs.

Moreover the infant papers treatment of diseases by essay in austria, gene transfer provides better result for a prolong period of massage papers time. It is the need of hour to discover new and cheap method of gene transfer technologies so to make the treatment of the diseases a little easier and affordable. Improvements in gene transfer are required in terms of transfection approaches to allow improved transgene uptake efficiencies. 1)Society of Applied Sciences. Gene Transfer Technologies and their Applications: K.H.KHAN.

Medical Biotechnology Division, School of Biosciences and hybrid paper, Technology, VIT University, Vellore-632014, Tamil Nadu, India. 2)Principles of gene manipulation: R.W.Old ,S.B. Primrose and R.M.Twymann ‘ sixth edition,chapter 10 and papers, 11. 3) BIOTECHNOLOGY 2020-From the essays zen buddhism suzuki Transparent Cell to. the Custom-Designed Process–Prof. Gerhard Kreysa Dr.-Ing. E.h. Dr.h.c.

Dr. R??diger Marquardt. 4) INTRODUCTION TOBIOTECHNOLOGY AND GENETIC ENGINEERING. 5)From genes to massage papers, genomes- concepts and applications of paper DNA technology–Jeremy W Dale, Malcom von Schantz. Search our thousands of essays: If this essay isn't quite what you're looking for, why not order your own custom Science essay, dissertation or piece of coursework that answers your exact question? There are UK writers just like me on hand, waiting to help you. Each of us is qualified to a high level in our area of expertise, and we can write you a fully researched, fully referenced complete original answer to your essay question. Just complete our simple order form and you could have your customised Science work in your email box, in as little as 3 hours. This Science essay was submitted to us by research papers, a student in order to help you with your studies. This page has approximately words.

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A for and against essay about the internet. Look at the essay and do the exercises to improve your writing skills. Massage Research! Do the preparation exercise first. Then read the text and do the other exercises. What's your opinion? Do you think the comparaticve internet is bad for infant massage research papers young people? For young people it is. They shoud be surervised while accessing the internet, because they might find pornographic content, violent content and such.

Internet, an invention which still amazes people in is own way, is not always good. It has really bad side effects on young adults. As in the essay, the young people get addicted to online games and essays zen buddhism they become addicted to the internet quickly. As we can see, there are many more online games being made like Pokemon Go, etc. is really dangerous for young people. I think it really should have a age limit. And also we must be strict about the massage research papers rules of age limit on internet too. Hybrid Car Research! I#039;m also a young adult but I find internet uninteresting and dangerous so I approve of it having age limits. For YOUNG PEOPLE yes it is. I think Internet is very useful in schools and in work. It#039;s also handy when you need to infant massage research communicate. On the essay other hand a lot of people are addicted from sites like facbook, twitter or instagram.

I also agree that learning on infant massage research papers the internet (like here ont the british council ;) ) is very usefull . So in conclusion internet is possibly the most usefull thing that we ever invented. :) I agree with all of you, but what about learning how to play a musical instrument? I think internet can be very good for teenagers because it helps them a lot especially for essays, projects and homeworks, but it can be also very bad because children spend too much time online. I like internet because I can listen to songs onYouTube and I use British Councile:) Hi BigBen6464. Page Paper! I'm glad to hear that you enjoy practising English here :) Is there anything in particular that you like on the site? Jonathan (LearnEnglish Teens Team) Thanks! I like your reading skills practice, stories about UK and What is it? in Study Break.

British Council really helps me with my vocabulary. Infant! I love it! I think the internet is an amazing tool which can be used for equally amazing things, but only in the hands of the essay for college right person. One bad aspect of internet is infant research papers that anyone could acess anything anytime. That means there is a lot of inappropriate content out there, but a good person is able to avoid it. Essays! In my opinion, though internet is very good and useful, but some young people use it for bad things.

Such as playing computer games and get addict with it. Many parents has banned the children not allowed to infant massage play forever,1 week or a day or so. But they never keep their word because, of course, parents love their kids, that is why after just a while,they let their children play AGAIN with their games, so really though, I think internet is senior useful with good children,and it is bad with bad children. I totaly agree. It all depends from parents.

If they let children to infant research papers spend too much time online children will get a bad habit when they grow up. You#039;re quite right! I agree with your good comment in point: #039;I think internet is bad grammar useful with good children, and it is bad with bad children#039;. !! :) In my opinion, Internet is one of the greatest people created. You have an access to billions of libraries from your computer, you are able to speak with your friends even if they are on the other side of the world. Many people work using the Internet. Now let#039;s mention disadvantages of the infant massage papers Internet and web-technologies. Firstly, many people nowadays become addicted and can#039;t survive for more than an hour without computer or smartphone.

They need to publish their photos in opinion essay racism in austria social networks, chat with 7 people in one moment and read new posts in their favourite online communities. Young people sometimes find their online-life better than the real one. The second disadvantage is that nobody can guarantee the safety of your personal data. Everyone has heard stories about hackers that published private and scandal photos of data base of the big company. It#039;s not really pleasant to know that somebody can easily find out papers all your secrets. To sum up, Internrt is a wonderful tool for searching the bad grammar necessary information, but social networks is quite tricky part of World Wide Web. I think the massage papers internet very useful for us .bcz we can get anything without spend alot of time in looking it . everything when we use it excessively and more than usually #039;it will be badthing. Well, we must admit that on this perfectly imperfect planet nothing entirely good or entirely bad exists. Essays! Internet is included. Surely it was meant for connecting people from all continents, but as the human mind has no border line we found out infant massage papers how to use it for different things that provide us either with information or amusement. It is said that the Internet is a good servant but a bad master.

Therefore, we can see that the problem isn#039;t in the Internet itself but rather in the humans, as it is us who are using it and who are asked FOR WHAT we are using it. So for our irresponsibility we shouldn#039;t blame the Internet but ourselves as we are unable to use a powerful tool for our own improvement but we use it for our own unwanted degradation. I think that#039;s quite right!! Nowadays, Internet has been the comparaticve outline most important thing in mutimedia life. Personally, l consider that the internet is bad or not,which depends on users. I think the Internet is something that we have to know, and we should know what is infant good and bad for us, and keep ourselves away from what can damage ourselves in any way. I think it is the same for essay young people, we have to research be responsible, because the Internet is a tool that helps us either with the school, or with any ordinary situation. i think internet is mla cover useful for infant research papers everyone. Senior Essay! If it was a bad thing, something awful, it would have changed instead of reaching the massage whole world. Is something crazy, used 24hrs a day, everyday of the week, a lot of information, and a lot of comparaticve outline, people online.

About teenagers who are addicted to games, that depends on each family. Parents could create rules, so no one would be in a bad position. And that also depends on the teenager#039;s sense of what he or she can or can#039;t do. It#039;s impossible to massage research papers live without internet in zen buddhism a society like nowdays, it#039;s just so easy to make a research, work on a project. c#039;mon, does anyone here wants to spend hours and hours looking for massage papers few informations? Internet is useful for everyone, for young and old people.

There#039;s a lot of sites that can help us a lot. Opinion! But, today Internet ha gone too far away. Infant Research Papers! Almost, there#039;s no home without the internet(more than 70% in whole world are with internet). Essays! Yes, there are a good and a bad sites on the Internet. Infant Research Papers! We have different web-sites(Facebook,Tumlr, Twitter, Instagram. ) that shows us a different world. We can learn a lot of things (English, French. ) and research paper a lot of other things. Infant Massage Research Papers! But we all know that Internet isn#039;t so much safe.Even if we think that we have good hide it our information, we don#039;t. There#039;s a lot of in austria, dangerous people in massage papers the world. To sum up, I completely agree with essay. No, i don`t thing internet bad for essays zen buddhism suzuki young people, because there is papers many information in the internet.

In my opinion, I dont think Internet is bad for people. Without the Internet, I cannot learn language (English) by myself and I wouldn#039;t be able to paper access to research papers lots of useful information that school doesn#039;t teach me. Research! On the other hand, internet is bad only when people dont know how to use it wisely. Massage Research! If young people use the Internet for studying and relaxing in proper way, internet would be very useful. To me, the internet#039;s the most wonderful tool that human had created. In my own perspective, the Internet plays an important role in comparaticve outline the communicating barrier. As you can see, people all around the world are using the Internet, including the elderly. We communicate with each other using the internet and it really helps us to stay connected with one another. Besides, without the internet, how are the countries going to develop? Our knowledge and the view to the outside world, the perspective to the outside of our comfort zone will be just limited, like a frog in the well.

So, why not? The internet is the infant massage best connection between an individual to the whole wide world. It#039;s definitely a boon. Opinion In Austria! Internet is very usefull for us. Infant Massage Research Papers! Internet is particularly useful for everyone, nearly all things are on the internet, you can get anything by only searching on it. Internet seems becoming really important nowadays, it helps people a lot, for example i use internet for learning English, reading news and doing research for comparaticve outline my tasks. However, internet also can give many bad effects, people become so fanatical on social media and often forget about massage papers time and everything they should do. I completely disagree that internet is bad for comparaticve essay outline teenagers, internet can be useful or useless, it depends on infant massage research the way people use the internet.

How does this photo make you feel? Can you write a caption for it? . Look carefully. For College! What's this everyday object? Play Wordshake and infant massage research papers see how many points can you get in 3 minutes. © British Council The United Kingdom's international organisation for cultural relations and educational opportunities. A registered charity: 209131 (England and Wales) SC037733 (Scotland).

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Class Member Links - OCS Class Hotel 515. Ted Aragon and massage research papers some of his brothers - Ted is on the right Now married to Melissa, we have a daughter Elena who is a Junior at Whitman College in Walla Walla, Washington. In 1963 married Maxine; Son Lowen, grandson Garrett, (Infantry Sergeant in the Army) ; Granddaughter Araya and grandson Avery Patrick. I am the third eldest in one family of essay for college fourteen (14) children; Ten (10) boys and four (4) girls; all still around. (Moms prayer has always been that non of infant massage research her children predecease her. My prayer has always been that mom live for a very, very long time.) 1967- Graduate: Bachelor of Arts; University of New Mexico. 1968 -Graduate: U.S Army OCS; 2nd Lieutenant. ** 1970- Graduate School of Economics: University of New Mexico.

1974 -Graduate: Juris Doctorate; University of Denver. Essay Outline. 1983- Graduate: Masters of Laws of Taxation; University of Denver. Infant Massage Research Papers. 1959-1967- Safeway Stores, Albuquerque, NM. 1967-1970 -U.S. Essay Outline. Army; Ft. Leonard Wood; Ft. Bragg; Ft. Belvoir; Viet Nam. 1972-1975- Staff Attorney; U.S.

Environmental Protection Agency. 1975-1980-Assistant City Attorney; City and County of Denver. Infant Massage Research Papers. 1980-1981-Landman; Atlantic Richfield Corporation. 1981-1984-Private Law Practice; Englewood, Colorado. 1984-1990-Legal Counsel; Texasgulf Minerals and Metals, Golden, CO. 1990-1995-Senior Attorney; Resolution Trust Corporation, Denver, CO.

1995-Present- Private Law Practice/Semi-retirement/Retirement. **After OCS assigned to bad grammar, a Construction Battalion at Ft. Bragg,; Rehabbed old barracks, also assigned extra duty as Trial Counsel (prosecutions under the UCMJ); Later assigned to Defense Language Institute at Ft. Bliss, Texas (Vietnamese Language Training); Thereafter assigned to MACV in Vietnam; Advisor to Public Works Chief of Gia Dinh Province; Pacification and Vietnamization Programs. Received Bronze Star and what I recall as the Vietnamese Medal of Honor ( Still looking for the award).

Photo at right: wife Mellisa, mother, Ted and infant massage research daughter Elena. More Ted Aragon photos from Viet Nam. Atkinson Cullin, LLC - Monroe, Georgia. Robert Bob Barnes is an established leader in the Texas banking industry with more than 30 years of experience. As president and CEO of IBC-Austin, Barnes oversees and directs the bank's activities throughout the entire Austin region.

He also serves as president of IBC First Equity, IBC's mortgage division, which specializes in home equity lending and second lien mortgages. In the summer of zen buddhism 2004, Barnes led IBC's official entry into the Austin market, which was marked by the opening of the infant bank's regional headquarters downtown at 816 Congress. (In 2001, IBC acquired Austin-based First Equity and made its initial footprint in the Austin market.) As part of essay racism in austria his leadership role, Barnes is spearheading IBC's expansion in the greater Austin area organically through new branch construction and research community reinvestment. International Bancshares Corporation, the parent of IBC Bank, is one of the largest banks headquartered in Texas. Currently, IBC operates more than 278 locations and 440 ATMs throughout South Texas and Oklahoma, and senior for college as a holding company, IBC has more than $12.1 billion in assets. As IBC continues to expand northward along the strategic I-35 NAFTA trade corridor, Austin and Central Texas will continue to infant massage papers, serve as key growth markets. Prior to accepting this leadership position, Barnes worked for opinion racism IBC-Houston for infant research papers three years as a senior vice president and commercial lender. During this time, Barnes honed his expertise in the areas of interim construction lending, mortgage banking and essays suzuki private banking. Before joining IBC, Barnes served in upper management with some of the Southwest's most competitive financial institutions, including Compass Bank-Houston and Weslayan Bank. Infant Research. At Compass Bank, Barnes was an executive vice president and helped lead the bank's residential and interim construction lending activity. He served as president of Weslayan Bank for six years until the financial institution was purchased by Compass Bank.

An active member of the Austin community committed to IBC's We Do More philosophy, Barnes serves as a board member of the University of Texas' McCombs School of Business, the Long Center Corporate Council and Opportunity Austin, an initiative implemented by the Greater Austin Chamber of Commerce's Economic Development Council. Barnes earned a Bachelor's Degree in Political Science from Randolph-Macon College in Ashland, Virginia and a Master's of Business Administration Degree from the senior essay for college University of Arkansas in Fayetteville He and his wife Laura have been married for 31 years and have three children: Katie, Adrienne and Harrison. He is an infant research, avid cyclist and gardener. My first duty posting after OCS and the orientation schools at Ft Eustis was as a Basic Training Officer at mla cover page paper Ft. Knox, KY until May of 1969. I then attended another school at infant massage Ft Eustis before I went to mla cover research paper, Vietnam in infant massage research papers August 1969. While in Vietnam I spent the first 6 months as a stevedore platoon leader in Vung Ro Bay unloading deep-draft cargo ships and LSTs loaded with PX items and a lot of bombs and munitions in primary support of the Tuy Hoa Air Force Base. Car Research Paper. The last six months in-country were spent running truck convoys about 80 miles between Vung Ro Bay and Qui Nhon, with occasional trips to Pleiku. I was the massage supply and property book officer in both companies- the one in Vung Rho Bay and the truck company in Tuy Hoa. I had to travel quit a bit to Saigon, Cam Rahn, Qui Nhon, Pleiku, etc which I usually did on either an AF C-130 or a Huey.

Sometime I would hitch a ride in a single engine Piper with an air observer. Those guys are crazy! In July 1970, despite the typical enticements of re-enlistment, I turned it down because I knew that I would come back to the states for a few months and then probably go back to Vietnam. I felt that I had been very lucky after having been involved in several truck convoy ambushes and did not want to essays, take that chance again, so I rotated back to the states and infant research papers was discharged at for college Ft Lewis, WA in infant massage research August 1970. Since my dad was in the Real Estate and Home Building business in my hometown of Owensboro, Kentucky I started working on homebuilding crews after I got back. I branched off in the fall of 1971 and formed my own homebuilding company in Jackson, Tennessee. When the economy soured in 1974, I got out of construction and I went into essays zen buddhism suzuki, management for infant papers The Wickes Lumber Company, first at the store in Jackson, Tennessee and hybrid paper a few years later at the store in infant massage Owensboro, Kentucky, my hometown. In 1973, while in Jackson, Tennessee I got married to Ann Sanders (a school psychologist) from Tullahoma, Tennessee and we had a son, Ian, in 1977. Paper. Ian is massage research papers now 36 and hybrid paper lives in Murfreesboro, Tennessee.

He worked for Outback for infant massage papers about 12 years but got out of the restaurant business and now works for his uncle's packaging and distributing business in opinion essay racism in austria Murfreesboro. In 1978, I resigned from Wickes Lumber to return to Graduate School full time where I earned an MBA in Dec 1979. In 1980, after Graduate School, we relocated to Nashville and massage research I went to work managing energy conservation and renewable energy programs for The Tennessee Energy Authority, initially, and essays then, for The Tennessee Department of Economic and Community Development after it absorbed the Authority. Infant Research. I retired from the department after 30 years' service in July 2009. I got divorced in mla cover paper 1985 and I have been single since then but have had several lady friends along the way- some of which I probably should have married and quite a few that I am glad I didn't. I have now been retired for almost 4 years and have been traveling quite a bit and continue to be active in my long-time, favorite hobbies of hiking, archery and infant papers shooting (pistol and rifle). Back in the 90s I traveled quite a bit around the southeastern US competing in both archery and shooting tournaments . I have also hiked lot in Yellowstone NP, the Tetons and here in Tennessee.

I still travel to racism, archery tournaments around the state but am not traveling so much to compete in rifle matches because that got to be too much work. I am a member of a small, private shooting club and we have a range in the Dickson, Tennessee area. We get together a couple times a month for infant massage research papers friendly matches. I am the secretary/ treasurer of bad grammar essays The Tennessee Archery Association and papers the Tennessee State Director for The National Field Archery Association. I am now gearing up for a Stephan D. Ambrose Historic WW2 Tour through England and Europe in September/ October 2013. I, along with one of my long-time shooting friends, have been talking about taking a trip like this for probably 20 years. We will be in research paper England, France (Normandy), Belgium, Luxembourg, Germany and research Switzerland for about 29 days total. To me, it is the essays trip of a lifetime. Buck graduated from Virginia Commonwealth University with a Bachelor's degree in Business Management in 1967.

He married Carol Brooks in July and entered the US Army in October 1967. In August, 1968, Buck was commissioned a 2nd Lt. He remained in the US during the infant research papers Vietnam War teaching Railroad Science at the Transportation School at Ft. Eustice. Essay In Austria. In 1972 He graduated from George Washington University with Master's Degrees in Personnel Management and Computer Science Management. Later he went to work for Electronic Data Systems (EDS) on Wall Street in NYC on the DuPont Glore Forgan account. Massage Research. In 1974 he transferred to Dallas, Texas and managed Employee Relations, Employee Benefits and Corporate Insurance. In 1980, Buck became Human Resources manager for the company's largest division. In 1982, Buck assumed responsibility for Productivity Services, a consulting division of EDS, specializing in work flow analysis and process redesign. In 1993, Buck joined EDS's newly emerging Management Consulting Division as a Managing Consultant. In 1996, Buck became head coach for High Performance Teams at EDS.

Carol is also a 1967 graduate of Virginia Commonweath University. After college she taught elementary school for essays five years in infant research papers Richmond, Ft. Belvior, and Newport News. Racism In Austria. She then embarked on massage research papers a career as a professional volunteer. Opinion Essay Racism. She is infant research papers currently director of the Community Food Pantry in McKinney, Texas, teaches Aerobics at the Princeton Community Ed Center and is President of the Princeton, Texas School Board. Jim Bodwell graduated from North Texas University and is now a District Sales Manager for Sysco Foods in Dallas. Essay. Son Stephen graduated from Texas AM University where he was Executive Officer of Company D2. He is currently employed as a Financial Officer for Maverick Capital, a Dallas based hedge fund.

Son John works for massage research papers State Farm as a Manager on the Disaster Team. In 1999, Buck retired from EDS and established PT Consulting Partners an essay, internet marketing firm. Buck plays golf, sings in the St. Peters Episcopal Church choir in McKinney, Texas and infant massage research papers walks 6 days a week 3 to 4 miles a day. Buck and Carol live in the country on 5 acres north of essay for college Princeton, Texas. They have 3 awesome grand children: Leah age 9, Jack age 4, and infant massage papers Ellie age 4. Fortunately, they all live in the Dallas area. After OCS and transportatiopn training at Ft Eustis, I spent 1 year at New Cumberland Army Depot in mla cover page research Harrisburg, Pa. managing a desk before going to Viet Nam in Aug.

1969. I was assigned to the 285th Transportation Company in Qui Nhen as a port operations officer with responsibility for supervising the discharge of Sea Land container ship and frozen food refrigation ships. After 9 mth. we were transferred to Da Nang. both very safe assignments. After returning from infant papers, Viet Nam, I went to work for Pan American World Airways training for 6 mths in New York working for 2yrs at LAX and SFO. This was the essays beginning of the decline for Pan Am and I was layed off in 1975. Prior to the layoff, I entered the MBA program at Golden Gate University in San Francisco graudating in 1976. Thank you GI Bill. In 1976, I married a Pan Am, flight attendent. the infant massage research okie from Muskogee. and moved to Tulsa in 1978. a great place to raise children and go to church on Sunday.

Christine was born in 1978 and Kurt in 1984. I worked a number of years in marketing for two large banks. In 1990 we purchashed Daniel Screw Products. a job shop making precision manchine parts to tight tolarances and customers blue prints until Jan 2013 when it was closed due to a lack of essay specialized expertise. During this time, Marie had two breast cancers and died in massage research Oct. 2010 of small cell lung cancer. Hobbies and interest over the past few years has included membership in Downtown Tulsa Rotary club and landscaping.

Bike riding and swimming has enabled me to play tennis 2-3 times a week on two knee-replacements. Opinion Racism. I have recently returned to playing golf because of infant massage a strong interest of hybrid my son. The interaction for a lot of papers us is amazing. After OCS I too went to transportation officer basic then on essays suzuki to Fort Rucker, AL.(General Staff) I was there for about six months when my orders came down that I would be going to Ft Lee, VA to attend Officer Supply School and infant massage research papers then on to Vietnam in late July. Mla Cover Page Paper. I was assigned to USARV HQ at Long Bien (ACS Comptroller's office).

I don't remember the General Dillard incident however. I do recall however being in the officer's mess a couple of times and sitting next to Col. Rheault (Green Beret) and couple of his officers who were being court marshaled for their interrogation of prisoners. They would throw a guy out of a helicopter while the other prisoners looked on. Believe the Col. Was found not guilty. After about six weeks at USARV I was sent to infant massage research papers, Cam Rahn Bay to II Corps general staff, investigating the general's non-appropriated funds, that is; officer, NCO clubs, PXs, etc. BG Delmar was my CG. Traveled quite a bit throughout the area but can't remember the names except Nha Trang. What amazed me the outline most with my military experience is the fact that I was never assigned to one MOS I was trained for, but to my college degree in accounting. Papers. That ended up being quite an asset, as when I interviewed for a field audit position at essays zen buddhism the Aetna Life Casualty Ins.

Co., the manager was a LTC in WWII and Korea. I was hired on the spot and spent 28 years there. Also worked for the Dept. of Revenue Services, State of papers Conn. for 12 years. Enjoying retirement in CT as well as at Cape Cod MA. In my spare time I make wooden cremation urns for indigent veterans. After OCS, I spent two months at Fort Eustis at essay Basic Transportation School and then Nancy and I took off for the San Francisco area where I was stationed at Oakland Army Base. We spent 6 wonderful months there and then in July of 1969, it was off to Vietnam.

I was stationed in Saigon with the 4th Transportation Command and we unloaded all the massage research papers cargo in the ports of Saigon, Cat Lai, and Vung Tau. I was not in any combat and Saigon was relatively safe while I was there. Was actually in mla cover page research paper Cambodia once (didn't know it at the time) looking for some lost cargo. When I returned from Nam in March of 1970, I took over a family retail grocery business in Bolivar TN. At one time the operation consisted of 4 stores, 300 employes and 15 million in annual sales. We got caught up in the Wal-Mart onslaught and Nancy moved to the Destin FL area in the fall of 2001. I stayed in Bolivar and tried to salvage the research operation but finally sold what remained and I moved to Florida in May 2003. Nancy is now the Executive Director of the Limited Partnership that owns the Sandestin Hilton Hotel and essay for college I am a Registered Representative of Lincoln Financial Securities, Member SIPC. I also offer life and health insurance to infant research, my clients.

Nancy and I have been married for 46 years and we have three daughters, Laura, Allison, and opinion essay racism Sarah. All three got married within 5 months in 2008. Picture above is our entire family--three daughters and massage papers their spouses and our four grandchildren. Creekmore Financial. Here is a little background summary of my life since OCS graduation. Before Vietnam, my first military assignment was at Ft Devens, Mass where I was assigned to the Provost Marshal unit and counseled diserters and AWOL service men who were in the stockade. Ft.

Devens was the holding facility and pricinpal stockade for New England. My job was to work with a military psychologist for senior prisoner assessment of infant research papers fitness for essay racism in austria military duty. Infant Massage. Many prisoners (enlisted and few officers) received 212 discharges from the service there. I married my wife, Linda, in hybrid 1969 and lived in officer housing at Ft Devens until August 1969 when I received orders for Vietnam. After serving in Vietnam as an aid-de-camp, I was discharged in August 1970 and went to work in research papers New York City and spent my career with several companies as a human resources officer and director. I retired from the Federal Reserve Bank of New York in hybrid April 2011. Linda and I have two children (daughter and son) and four grandchildren ( 3 boys and a girl). We have lived in Kinnelon, NJ, a NYC suburb, for 35 years.

My family spends the summer at Point Pleasant, NJ. (I'm currently managing the rebuild of my Dad's home (he is 95) in Point as it was flooded during the Sandy Superstorm.) Linda and massage papers I like to comparaticve essay outline, travel in the U.S. and Europe. In Feb 2014 we are taking an 18 day trip to Thailand, Cambodia and Vietnam. I want to show her where I was stationed with the First Logistical Command (Vung Tau) and USARV (near Siagon), and see how the infant area has changed in 40 + years. Stu and Sandy Eaton -beard and mustache now gone. The candor and humor of my classmates in comparaticve 515H astounds me - the stories are great!! I am not sure if I am unique, but I didn't appreciate it then nearly as much as I do now!! The detailed memories of those who have posted memories is great!

I am also so happy that it appears that all of us made it out alive (and hopefully whole) from our Viet Nam experience. Some of us have passed on since, which is unfortunately how life goes. Research. I am hopeful, that we are enjoying, or at comparaticve least anticipating, retirement. I look forward to our reunion!! Many thanks to infant research papers, Buck Bodwell, Jeff Fishman and Rob Creekmore (and possibly others) for their initiative to get this going!! There will be rewards @ the reunion for you from bad grammar essays, many of us, I am sure!! Hopefully what we all learned at Ft Belvoir and the rest of our time in the Army paid many dividends to us through our personal and work lives. Massage Research Papers. One dividend I enjoyed was that my military experience and background was a key factor in securing two jobs, especially the position I retired from after 25 years.

The hiring authorities appreciated and respected my service in opinion racism the military. Following our graduation from OCS, many of us attended the massage research Transportation orientation course at Ft Eustis. This was a country club compared to our time at Ft Belvoir. My first PCS was Japan at Camp Zama, at Headquarters USARJ in a staff position, totally unrelated to Transportation. Fortunately, after about six months, I was able to hybrid paper, transfer to infant papers, Transportation Directorate. As (good) luck would have it, the job was as Assistant Air Traffic Coordinating Officer, responsible for hybrid car research supervising the air freight section. I lived and worked on two Air Force bases, Tachikawa and Yakota Air Force bases. The accommodations and facilities were far superior to papers, Zama, and my BOQ was about 25% flight nurses who mostly flew medevac flights to and from Viet Nam and the States. Essays. My office was right on the flight line at Yakota.

I would go out to the flight line when Viet Nam charter flights came thru to see if I recognized anyone - never did though. It is highly likely that many of you who served in Viet Nam spent a short time at Yakota on a refueling stop! I got married in Japan, not to a nurse, but a teacher in the dependent schools. Additional information from Stu Eaton. 1.As those of you know who were in the Nam it gets very hot! So when I was with my transportation company in Chu Lai, I always slept in the nude with a loaded 45 under my pillow. One evening, someone was shooting up the massage papers company area and so I straped on my 45 and went running out into the compound to find who it was. I saw a Sgt. E-6 and car research asked him who the infant massage research papers hell was shooting up the bad grammar area and he swung around with his M-16, pointed it at my head and asked who the hell wanted to massage papers, know. Well, obviously I didn't have to look any further or ask anymore questions. As a matter of fact I thought I was a dead man.

The Warrant Officer in my hootch saw what was going on outline and called to the Sgt. by name to distract him from me. The WO jumped him in the hootch and knocked him out. The funny part is that my men busted my chops for months about standing absolutely frozen with nothing on but my little 45. Infant Massage Research. Wish someone had taken a picture! 2. After the CB's built a basketball court in our company area, we used it for all sorts of things. One day, my platoon and I were playing baseball. We had played a number of innings when someone hit the bad grammar ball and infant it went under my hootch. Right under my room. One of my men crawled under the paper hootch to infant research, get the ball and didn't come out for bad grammar essays quite awhile. Infant. I finally said, you must have found the ball by car research paper now, let's get on with the infant massage papers game.

He nicely said that he wasn't going to move because there was a booby trap planted under the floor where my cot was with 20 lbs of explosives. The MP's were called and OD. It turned out that my hootch maid was V.C. Hybrid. I guess she didn't want to clean my room anymore. 3. Now for you Engineers. We were on a convoy and the road had been swept by Engineers hours before. One of my trucks hit a mine and was totally blocking the road and so the convoy came to a dead standstill, which of infant massage research course we hated. The Engineers came back in to re-sweep the road and guess what guys, They found an unexploded mine under the rear right wheel of my jeep. You aged me 50 years.

4. I had never been through an I.G. Inspection but the word came down that our battalion was going to get one. As you know no unauthorized equipment can be in your unit or it is a complete failure. We had unauthorized gun trucks, hundreds of weapons and equipment. Essay. I always carried with me on convoy, 1 M-16, 1 45 pistol, 1 38 pistol, a sawed-off shotgun, an AK-47, a grenade launcher and an M-1 carbine. I always thought I was Audey Murphy. Our CO, Capt.

David Oates was on old line soldier and new exactly what to do. He had the Engineers dig a hole in back of infant massage research our company area, 80 ft. by 50 ft, by 12 ft. deep and in austria you guessed it. We buried everything until the inspection was over including the massage gun truck. He was a piece of work. We passed with an excellant grade. We did lose points for dirty trucks which had just come back from a convoy.

5. Now, I will tell you about a really stupid Colonel who commanded our Battalion. Bill Shugarts will back me up on this. When we went a really dicey convoy we were escorted by some gunships and lochs. TheColonel was upset because he couldn't tell which jeeps held his convoy commander,or asst. convoy commander, etc. So he decided that he would put bright pink 6 ft. panels with bright yellow numbers on the jeeps so he could see them from the sky. He put #1 on the Convoy Commander, #2 on the Asst.

Convoy Commander, etc. Of course, the Viet Cong being so stupid would never fighure it out. I argued with that man to no avail. What a jerk. Two miles into comparaticve, the covoy a strange magical strong gust of wind blew all the infant papers panels off the jeeps. I'm sorry to hybrid paper, say we could not stop to retrieve them. Steve Furste is a retiree of Dow AgroSciences where he served as corporate Community Affairs Manager in the Government Public Affairs Department for 10 years. Furste began his career with Elanco Products, a division of Eli Lilly and Company in 1980 where he held sales and marketing positions. . In 1984, he accepted a position as a clinical research associate in the clinical trials area of Eli Lilly and Company, where he remained until 1990.

In 1990 he joined the Lilly-Dow joint venture, DowElanco, and held various management positions until his retirement in August of 2004. Furste has been the Executive Director of the Boone County Habitat for Humanity affiliate since 2005. A native of Fort Wayne Indiana, Furste received a B.S. degree in infant research agronomy from Purdue University in senior essay for college 1967, and received his MBA from the research papers University of Utah in 1972. He served in the U.S. Army from 1968 until 1972, during the Viet Nam conflict and was discharged with the rank of Captain. Comment: I am presently executive director of Habitat for Humanity of Boone County (since my retirement from Dow Chemical) and our Habitat affiliate became the senior essay first in Indiana to build a home for massage papers a wounded warrior in 2012(a young Marine sergeant who was hit by outline a roadside IUD while in Iraq.

I have a wonderful DVD of the military celebration of his new home and research will send it to anyone interested in essay for college seeing it - have your kleenex ready when you watch it . steve. A lttile more on my experiences after OCS: I spent another 8 weeks I at an Engineer class and roomed with our old TAC officer - Peter ? forgot his last name. From there I got orders for Germany (I was VI- vol indef ) and I landed in Germany on the French boarder at research a place called Zweibrucken. Sat behind a desk for 4 years as a logistics staff officer and was discharges in 1972 as a Captain. I married a girl from my home town and my daughter, Jennifer, was born in hybrid paper Germany. I went to infant massage papers, night school and got my MBA as there was a program for junior officers from the 4 branches and I did it over 4 years going to classes twice a week at night - isn't the GI bill great! I spent 4 years in New England designing irrigation systems for golf courses and then was with a JV in the Cayman Islands until 1979. I spent the last 25 years with Eli Lilly and car research Company and Dow AgroSciences here in Indianapolis. I am a Dow Chemical retiree as I retired in 2004 and was promptly hired by research papers Habitat foe Humanity in our local county. Page. I love it and it is a great way to give back. Steve.

I spent the two remaining years of massage my Army commitment in essay outline the USA. I'm not good at infant massage maintaining friendships, and I kept up with not a single fellow OCS member once we left Belvoir. I assumed all along that I was one of the very few in our class, and perhaps the only one, who did not go to senior essay, Vietnam. I can't say I felt deprived, but I often wondered what had happened, and infant research how many did not come back. Essay. This effort by Buck and others to massage research papers, reconnect has been just wonderful, opening a door to a period in the very distant past. Thank you, and I really mean that.

I left Belvoir, rebounded immediately from 6 months of hell by buying the first car I looked at, a red Porsche (which I'd totally forgotten about until Tom Dilatush inquired as to whether I still had it, no, it's long gone, I now drive an old Subaru). Senior Essay. All my worldly possessions fit nicely inside it, and I headed out to Fort Benjamin Harrison in Indianapolis, home of the massage research Finance Corps, affectionately known as Uncle Ben's Rest Home, and site of the Finance Officer Basic Corps. Two months of classes, mostly about how the bad grammar Army pay and benefit system worked. The assumption was a few months stateside, then Vietnam. (Continued via link below) Good for you guys, putting all this together. After this long you have to look close to recognize yourself. I changed my phone number on the excel file to research papers, indicate my cell phone, as my home phone will change when we move in a few weeks. I added my wife's name also. Bad Grammar. She actually was able to visit me during OCS as I got a couple of passes to go into DC.

Tom Richards (TAC Officer) wanted to be a pilot and my future wife at the time was a flight attendant, so he was very accommodating. Papers. My home address is the new one and is O.K. as we already have the house. Hybrid Paper. My email address will remain the research papers same. I have lost track of everybody. I do remember the class at in austria Ft Eustis. I was stationed at Ft. Bragg and Ed Maier, who was in 2nd Platoon, was my neighbor on post. We enjoyed goofing around with Ed and his wife for that year and then we lost touch.

I ended up in Korea and missed Nam. Not sure how that happened, but a lot of guys out of Bragg went to same place I did. Infant Research. I'm still working at bad grammar essays my own consulting company (Preferred Marketing Programs). Infant Massage Research Papers. Good to bad grammar essays, hear from you guys. Infant Papers. I'll be happy to stay in essay racism in austria touch. After reading the massage Bio's of some of my classmates from the OCR, I realized that each of us reach a point in our lives that many would call a Defining Moment. God doesn't reach down and touch us on mla cover page research our shoulders and infant research say this is crucial point in your life and how you react and essay overcome the obstacles that are before you will have a tremendous impact on how you live the rest of your life. Certainly, being a member of Class 515 Hotel was such an experience for research me. I came from a different background than most or probably all of you. I haven't lived at home since I was 13 years old.

I attended the Mount Berry School for Boys in Rome ,GA for most of my teenage life. Berry was a unique experience that probably had as much impact on my life as OCS but in a different way. All of us were poor, not just broke, but in dire straights, hence the reason for us being at Berry. At a recent reunion, one of my classmates said we were sort of hybrid paper like a Band of Brothers. I chuckled and told him I endured more in six months of OCS than I did for the four years I spent at Berry. He looked at me like I was nuts but I assured him the guys I graduated with from OCS were a special group of young men. Most of papers us weren't the essays smartest (Peter being the exception), or the strongest, or the best athletes but we all had a common bond that stood us through the tough times at the OCR.

One, we wouldn't quit, and two, those of us who graduated on massage research papers that August day in 1968 were team players. We looked out for each other! The old adage Cooperate and Graduate was often heard during our initial training but truer words were never spoken. 10 years in page research paper Atlanta the 70's (even roomed with Kirby Atkinson before he married and moved to New Orleans) Worked as an accountant, which was not for research me---Then field sales representative to comparaticve, the chemical industry--Left that job and research traveled (busses, trains, boats, and hitch-hiking) through Central and South America, ending up in Bolivia. Opinion Racism. Then graduate school (MA Public Administration), while in graduate school I worked as a waiter and infant massage research papers became engrossed with the restaurant industry.

10 years in mla cover paper Washington, DC (those wonderful Reagan Years) as general manager of restaurant in Chevy Chase, Md. The restaurant industry is insane for infant massage research papers someone who is a day person (late nights, alcohol drugs). Left and traveled around the world for essay 11 months, ending up in Paris where I parked my dead ass for several months. Infant Massage. Eventually I had to return to the U. S and get on with my life. Back to Atlanta where I discovered horticulture volunteering at bad grammar the Atlanta Botanical Garden. After a year of volunteer work, I decided if I wanted to be something in that field I need to get a degree------Therefore, Hort. School at the University of Georgia, where the infant massage research students were young enough to be my children (I was 48). Great time living in Athens, Ga. for three years. Then back to Atlanta (1995) and a job as the Grounds Superintendent at opinion essay racism in austria the Atlanta Botanical Garden. That was (is) my career and my passion.

Never been married, well not in the traditional sense, but have had a long-term relationship, but am now single in infant massage papers my old age. Read between the lines----I am Gay and want to be married again. This time legally in the United States. There are other things I could write, but that's enough or more than enough. I can also understand the dislike of dealing with mines since, with my EOD MOS, I spent a year running a mine sweeping platoon in Vietnam (think Hurt Locker without the big suits), and, on the whole, I think that I preferred OCS. Photo of our new it can't fail 3/4 ton Mine Detecting Vehicle after the first day of use. The problem seemed to be that it would detect only one mine before becoming unusable.

So we went back to the hand-held metal detector and keen eyesight method. I also did the Ft. Dix, Ft. Knox route before OCS - maybe that's how we all wound up in the same class. Went to Ft. Benning for jump school after Ft. Belvoir, then a stint at opinion essay racism in austria Ft. Bragg with the 82nd Airborne Div., and, then, an extended tour in RVN. Ironically, I shipped out to Vietnam two weeks after attending Northern Warfare School at Ft. Greely Alaska - typical: Army appropriate training -two weeks at minus 30 degrees F before heading for the jungle. For a brief moment - as I became a short-timer - I thought about extending my enlistment in the Army, based on offers of research promotion and advanced military-based educational opportunities, but finally decided to return to Philadelphia and my alma mater, the University of Pennsylvania, to suzuki, pursue a Master of Architecture degree.

In hindsight, that decision turned out to be especially fortuitous since I not only received a fantastic graduate education enhanced by the opportunity to study with one of the greatest American architects, Louis Kahn, but, more importantly, I met the person who would become my wife, Cecily Corcoran. Perhaps some of you will understand that having just returned from the jungle and my Thunder Runners minesweeping platoon duties, my social graces were somewhat rudimentary if not atrophied, and it was Cecily who took me under her wing, helped me become a normal person again and returned me to civilian life. This September we will celebrate our 40th wedding anniversary, and I can't believe that anyone is more blessed than I am to have had such an extraordinary partner in life. Trained as a landscape architect, Ceci is currently Executive Director of the Agua Fund, a Washington, DC based philanthropic foundation, which focuses on advancing the massage environmental and social well-being of particular regions of the country and essays zen buddhism suzuki is a member of many environmentally oriented organizations including the National Consultative Group on infant research Biodiversity and the Chesapeake Bay Funders Society. We have two wonderful sons, Ned, who is finishing a MFA degree at UC San Diego and Tom who is living and working in Philadelphia. After graduation from OCS went to flight school. Essays. Then a year in infant research Vietnam with the 220th Recon Company (Catkillers)flying the 0-1 Bird Dog in I Corps out of Phu Bai (Dec 69 -Dec 70).

After five years in the service went to work with Ryder Systems and spent most of my 33 years in Raleigh, NC. Have been retired for essay outline the past 10 years living in Clarksville, VA. Massage Research Papers. Play golf, fish, ride a Harley and work on this old House built in 1829. Also, spend time with my two twin grandchildren. Good to hear from my OCS friends after all these years. Dean. All of your stories are impressive.

Class 515H along with the TAC Officers were a great bunch of teammates who all worked together to make us tough enough to racism in austria, survive the perils of Military life. My hat goes off to all of you. The total experience of going to Basic Training, Advanced Infantry and then to be selected with the elite few to attend OCS made all of us realize what we were all made of, what we could endure, and to teach us how to infant massage, respond for the safety of paper our Military Personnel whom we would be put in-charge. During the process of weeding out and final review in front a Military Officer Selection Committee at Alameda Navel Air Station I was with a group of fifty plus volunteer civilians who recently joined the Army and after final testing and oral interviews were over I and three others were told to leave the room and infant massage research papers would be told if we were accepted or to be sent home for essays zen buddhism suzuki various reasons. Infant Massage Research. After waiting for what seemed eternity, a Sergeant entered the hybrid car research room and said that we were the only ones that were accepted through the selection process and were to fill out a branch and infant papers dream sheet. Transportation Corp was my wish and essay racism my dream sheet was the West Coast or Germany for my long tour and then the short tour was a place that was little known to me called Thailand. I said to my self Fat Chance. I was sent home and was to report middle of October, 1967. I reported as ordered and during the Physical process it was discovered by Army Doctor's that I had an ear infection and a broken bone in my left foot.

They stamped me 4F. My heart sank and research thought all of the testing and interviews was for not. I stated that my left foot was no problem and I wanted to go in the Army since I had been drafted 3 previous times and received a student deferent 3 times. The Med Specialist then said I was to report back in two weeks after my ear infection was gone. Outline. I went to a doctor who stated I had MONO. I ended up with an infant papers, ear infection that would not go away and had to get my tonsils removed. I reported back in two weeks and the Med Specialist stated he needed to inspect my ears but could not find his scope and asked if I felt OKAY.

I said yes and he stamped me 1-A. Comparaticve Outline. I was sent to Fort Lewis for Basic and later to Fort Ord completing Advanced Infantry Training. Papers. Friday's graduation brought a surprise, I was informed that I had lost my position at TC School and would be reassigned. I asked what my options would be and personnel stated that there were openings in several branches, ie. Infantry, Armor, and comparaticve essay outline the list went on to Engineer.

I said I would accept going to Engineer OCS, and the following Monday I arrived to Fort Belvoir. After leaving Fort Ord, I always received my Orders late for some reason or other. It made life interesting and the old Military saying Hurry up and massage Wait was true. During the last couple weeks of OCS, most fellow class-members had reveived their assignments for weeks, except a hand full of us who were held back in suspense. Comparaticve Essay Outline. I remember the massage papers very last week of class the remaining hand full of in austria candidates were informed by a Personnel Officer that since previous classes had turned down 17 European assignments the hand full of us left were all going to Europe as our next Duty Station. After the August Graduation in massage papers 1968, I attended TC School Orientation at FT. Eustis and essays then was provided orders to be assigned to the 110th Transportation Company with an APO address but no physical destination. What a mystery. After OCS I was stationed at Ft. Meade, MD, then went to language school. In Vietnam I was with Advisory Team 45 in papers Ninh Thuan Providence where I did a little of everything.

As soon as I got home I started law school in my home state of Montana and reenlisted as the opinion in austria executive officer with a local Army Reserve engineer company to help pay the infant bills. For nearly 30 years, I practice law in Montana and essay for college spent time as the dean of the law school. Along the way I served on the board of a local hospital, then the board of the system it was part of. In 2000 I was asked to massage research, join the system as an employee, working primarily on organizational issues and leadership development - not law. I currently serve in a similar role as senior vice president of Providence Health Services, a not-for-profit system that has thirty-two hospitals, 350 clinics, non-acute facilities, housing and educational institutions in five western states. We moved from the quiet of Missoula, MT to essays zen buddhism suzuki, Seattle in 2007.

While I occasionally envy friends who have more control over their schedules in research retirement, I continue to enjoy the paper challenges of the work, the colleagues I work with, and the organization that has been serving people here for over 150 years. On the personal side, Maggie and I were married only massage research a few months before I left for Viet Nam. We still shake our heads about that. We have been blessed with three sons and six grandchildren, all of whom fortunately live within driving distance. That means we travel quite a bit to spend time with them. The photo was taken at our 2012 family reunion with our crew and a friend. I may have been the luckiest of all because I was turned back from our graduating class, sent from my first assignment at Ft. Bragg to Defense Information School in Indianapolis landing in comparaticve outline the Public Information Detachment for the U.S. Army Engineer Command at Long Bien. I entered a talent contest in Saigon at the urging of my commanding officer (a captain) and spent 30 days as commanding officer, MC and standup comedian touring the country with a group of soldier/sailor entertainers visiting units throughout South Vietnam.

For the papers rest of essay my tour I worked out of an air-conditioned office, traveled from the massage papers DMZ to the Delta visiting our engineering units and hybrid supplying stories for the KYSU (Engineer in massage papers Vietnamese) Magazine we published along with our weekly newspaper and worked with Armed Forces Radio/Television in Saigon (sorry about that Ho Chi Min) on the production of comparaticve films. I wrote, edited and narrated films on Land-Clearing Units and the Lines of Communications asphaltic highway project which were broadcast on Armed Forces Television. I entertained my fellow officers at research papers night by getting the base vector control folks to give me the rats and shrews (unlike our American shrew these suckers were huge) they trapped around the essay outline PX's to feed to my two pet Burmese Pythons. It was better than television after a six pack or several scotch and waters. I became commander of the unit as a First Lt. in March of infant papers 1970. On the sobering side, my commanding General John A. B. Dillard had asked one of my men (an artist/photographer) to accompany him on a flyover inspection of asphaltic highways near Pleiku. He was bumped from the essays flight (one lucky dude) on a Huey Slick because too many Colonels and Lt. Colonels wanted to rub elbows with the Major General.

They didn't have a Cobra escort (which was supposed to be the infant massage research papers case whenever a general officer flew) and needless to say they flew below the three thousand foot level staring in hybrid car research awe at a 24 foot wide asphalt road. General Dillard, our Command Sargent Major and everyone on board were killed when they came into the sights of a Chinese 51 caliber machine gun. Research. Dillard was an outstanding General and essay outline a good and decent man. His temporary replacement Brigadier General Tarbox (I'm not kidding) made General Halftrack of Beetle Bailey fame look like a military genius! He was a fellow classmate at West Point with Dillard yet he made me write all the letters of condolence to the grieving wife and daughter with the boxes of personal items we shipped back to infant massage, the states. For two or three weeks after the funeral, which was recorded on audio tape with my narration, (thanks again Gen. Tarbox) more pairs of socks and underwear would show up and I had to write another ghost letter which he would review, making two or three tiny changes then mail back to the family. I was one lucky SOB and opinion essay racism because I was single at the time and felt I was indestructible even with a near miss in one of the research monthly rocket attacks on Long Bien or missing a firefight by five minutes. I was visiting one of our land clearing units to outline, participate with our D-8 bulldozers equipped with Rome Plow blades as we did a cut chewing up 500 year old triple canopy forest (maybe this is why I'm a bit of an environmentalist these days). After the cut was completed I hopped off the infant massage papers APC that provided security for the bulldozers and on the next cut enemy contact was made and a firefight ensued. You just never knew.

For me, there is a catharsis in suzuki recalling these experiences. In one way or another they shaped my life for good or ill. In my earlier email my uneventful career was meant to massage, only to describe my two year stint at Ft Meade. It was embarrassing, but my biggest challenge was sometimes driving in essay outline Baltimore Wash parkway in rush hour traffic to infant massage papers, make reveille after a late night. I got to live off base in a fun area so getting up at times to be timely was sometimes a challenge. After the Army things sped up quite a bit. Essays. A few jobs in infant research papers sales and then I started a novel business which would have a good 30 year run. I was lucky and sold it in 2004 before economy tanked . It was hard but an exciting ride -plenty eventful.

I think the Army/OCS training some how sunk in essays zen buddhism and was a big help to stay the research course when going got tough. I started the business when I was 32. I was single at the time; 6 months into the start up, I ran great woman, a divorced mother three children. We got married and 2.5 years later I went from outline, being a single guy with 2 roommates to having a wife, 5 kids, a mortgage, and a pet rabbit. Post Army life definitely very eventful. Infant Massage Research Papers. Greater than the opinion in austria successes in business was surviving as a step parent and infant massage research and winding up with a coherent happy family 35 years later . About four years ago, after being mostly retired ,I stumbled into a project that gives me purpose and opinion in austria keeps me occupied as a volunteer. Massage Research. It also reconnects me to the Army. I now spend about 20 hours a week as a volunteer with a team lobbying to make a very simple technique to help relieve PTSD and to build/restore resiliency in both soldiers and vets evidensed based.

TRE TM is technique which is affordable and very powerful tool for help recovery from chronic or traumatic stress or just to make people feel good. With others, we have successfully lobbied the opinion racism Army and Department of infant massage research papers Defense to call for clinical trial proposals and then made proposals for a controlled study; we believe that clinical trials are imminent and finally seeing the training working its way into essays, the into VA facilities even before the validation studies. This has a labor of love it is fun and massage research papers rewarding. This weekend, we just celebrated our 50th high school reunion. Peter is senior essay right; no one really cared this weekend about infant massage research papers, anyone's resume, bank accounts , or achievements. We were just happy to suzuki, see each other and to infant papers, hear people's stories and takes on bad grammar life. Sorry if I have rambled so long; I look for ward to hearing people's stories and takes on life . Receiving all your emails since Bob Barnes contacted me has been just amazing. Infant Massage Papers. It's touching to hear what classmates like Bill Oster and Bll Shugarts are doing to give back to the men and mla cover research paper women who voluntarily defend and serve our country. Most of my business career has been in the defense industry, primarily military resale.

Corporately, we are involved in programs that benefit the massage research papers Military Family Association, Fisher House and Wounded Warrior Project. We also have an employee who is an accomplished professional fly fisherman and has linked us with a terrific program called Project Healing Waters, training Wounded Warriors with amputations in fly fishing. Hearing Jeff's story of how he got to the 101st Airborne reminded me of how I arrived at my assignment in essay Vietnam. I flew over infant massage research with two Air Defense Artillery Captains who told me their orders were for 8th/4th Artillery Battalion at bad grammar Dong Ha Combat Base, the northern most point in South Vietnam. They related how they would have to infant, live underground being within artillery range of North Vietnam as well as subject to many rocket and mortar attacks. My orders only read Cam Ranh Bay. When we arrived, they spent the night and went back to the airport to fly to Da Nang. Cam Ranh Bay being an RR center, I told them to come see me if they got RR down here.

Later that day I got my orders - Da Nang Support Command. I went to the airport and flew up to Da Nang, with these two Captains. My orders read Da Nang Support Command, and, there being RR also at Da Nang, told them to come see me if they got RR here. I reported to Da Nang Support Command, and opinion racism a couple days later got my final orders - Dong Ha. Took me a few days to infant massage research, get up to senior essay for college, Dong Ha but became Lt. RR as soon as they saw me. They did initiate me into their unit - which involved drinking an 8MM howitzer shell filled with the infant massage research alcoholic beverage of my choice. Essay In Austria. While it wasn't quite as bad as they had advertised, like most of you I had many experiences. When I arrived in Vietnam, the 1st Marine Division was standing down and coming home, and we (Army) were taking over defense of the DMZ.

We took over the Artillery and Armored Cavalry missions, and the ARVN's took over infant massage research the Infantry mission. Could write a book on Vietnamization. We were very familiar with the mla cover paper land mines and unexploded ordnance. The Marine Corps had big ammunition facility on massage research the southeast side of Dong Ha Combat Base that was hit and virtually destroyed by NVA Artillery, either in Tet of 1968 or earlier. It was left abandoned when we arrived in 1969 but marked to keep anyone out. At some point during my year on the DMZ, we had a visit from the Deputy CG for essays Support for Vietnam who flew over the area on his way in and saw some things he wanted identified. A couple of our troops knew a way in and out and my fellow Lieutenant and I were able to infant research, go in and retrieve what turned out to be bags of essays grass mulch (hard to believe) to satisfy the General's curiosity. There were are huge number of unexploded artillery shells in there. Can only imagine what it is like today.

I also remember the infant massage research papers Catholic Church - I believe in Quang Tri or Hue - shot up in racism Tet of 1968 also. For those of you in massage research papers our Armament School class at Aberdeen Proving Grounds, you may remember one of bad grammar essays our instructors, Master Sgt. Infant Papers. Johnson. Sgt. Johnson served in our unit in Dong Ha and was a rock with the comparaticve essay troops - I am sure much like the Platoon Sgt Jeff served with. We all share an experience that impacted and research papers shaped our lives that we could not have received anywhere else! Can't thank enough Bob, Buck and essays all the others that have put this effort together to re-unite our class. Laurel and I have been happily marred for 30 years now since we met in research papers Denver.

I will give you the short version of the journey to New Mexico. Upon graduation from OCS, I had extended to Volunteer Indefinite status and was posted to Germany assigned to bad grammar essays, one of the Maintenance Battalions that services the infant massage research papers huge stores of equipment pre-positioned in case of suzuki WWIII. Infant Research. Not much fun. On the second anniversary of our Graduation, I received a promotion to Captain and orders to Vietnam. I served initially in Long Bin as the Ship Officer for the Collection, Classification and Salvage (CCS) point for the southern half of Vietnam. Essentially I ran the junk yard.

Not a bad gig, as we could trade salvaged parts with the bad grammar essays Aussies and Kiwis to help them keep running the US Jeeps they had stolen form us. Infant Papers. They traded steaks they had stolen for the General Officers Mess. Subsequently, I was made Company Commander of the 548Th Light Maintenance Company - Direct Support. Immediately we were reinforced to full strength and sent to the village of Dong Ha near the DMZ. Our initial mission was to support 1st Brigade of the 5Th Mechanized Infantry Division and comparaticve essay the Vietnamese when they went into Laos to interdict the Ho Chi Min trail. To do this I took a large detachment to our forward base at Khe Shan. Yup, the same place a Marine division was pinned down during Tet '68. A interesting place - all the hilltops look like golf balls from the massage research 1000 pound bomb craters from the B-52 strikes. After the North Vietnamese kicked the senior for college South Vietnamese out of Laos their withdrawal turned into a dress rehearsal for the 1975 collapse when they tried to withdraw from the infant massage research papers central highlands. Bad Grammar. Result was that we had to bug out fast.

It was about research, that time we captured the only prisoner of war for the Da Nang Support Command. We had a NVA Lieutenant give up inside our perimeter. Freaked my troops out as he just stepped out essays of the elephant grass along the path and flagged down their jeep. They decided he was NVA because of the AK 47 and no boots. After Khe Shan, we returned to infant massage research, Dong Ha but had no mission. It was frustrating to essay, watch a good unit degrade into a bunch of juicers and druggies. Eventually, orders came that the 548th was being withdrawn from Vietnam.

Orders also came that my company was being Flag Changed to the 528th So the troops in massage the 528th went home and hybrid car research we stayed in massage papers country. Still no mission. Essays Suzuki. At this time , I decided to request relief for active duty a leave the army. I was tired of infant not trusting my troops and finding hand grenade pins on my bunk as a warning from the drug users. Finally, orders came to essays suzuki, flag change the 528th to the 185th . All troops with less than 60 days left on their tour would go home. We went from Dong Ha to Quang Tri and finally to Da Nang and home. I still have the flag!! I also have a pretty extensive slide and massage research papers photo collection of these various bases. After the Army, I returned home to Pittsburgh. There I went to work for H.J.

Heinz in hybrid car research paper logistics. Interestingly enough that was what the Army trained me for - Depot level supply and logistics. I also was able to earn my MBA from Duquesne University. Infant Research Papers. Over the course of my 30+ year career in the food industry I have lived in senior essay Pittsburgh, Denver, Detroit, Denver (again), Southern California, and infant massage Chicago. In 2004, I exited the Corporate world.

Having decided the own and opinion essay operate my own business, I eventually settled on JAN-PRO Cleaning Systems as a relatively risk free franchise opportunity. Research Papers. We moved to Rio Rancho in 2006 to start up the essays zen buddhism business. Laurel is President and majority owner and handles Admin, Accounting and Franchise Sales, I handle Account Sales and Operations. In the research papers 7 years of operation we have grown sufficiently to hire a General Manager and zen buddhism suzuki are now in infant massage research Semi-Retirement. Our primary home is here in Las Cruces but we still have our Rio Rancho home. We commute to spend 3 days a weeks in Rio Rancho as I still President of The Rio Rancho Rotary - Sunrise Club. Hope this didn't bore too much.

JAN-PRO of New Mexico, Inc. 2320 Grande Blvd. Suite E. Rio Rancho, NM 87124. Robert G. Rauscher (Bob) of bad grammar essays Lakewood Ranch, Florida has joined American Property Group as a Commercial Real Estate Professional. He is originally from Skaneateles, NY and a graduate of Syracuse University. He served 4 years in the US Army achieving the rank of Captain while on duty on Vietnam where he received the Bronze Star and Vietnam Commendation Medal. Bob has excellent credentials to be successful in infant massage research papers commercial real estate. Paper. He has a 25 year background working for a Fortune 500 Company in Vehicle Leasing and Transportation Logistics. He held various positions including Director of Marketing and Sales Development, Regional Sales Manager, and infant massage research was a member of senior essay their exclusive Roundtable Sales Achievement Club. I lived in a BOQ at infant massage the other end of mla cover paper China Beach in 1970-71 and infant massage papers we were next to the Marble mountain Helicopter base and got so much incoming rocket attacks that were fired at the base that went long into our area that I switched to night duty.

Also I had a 2 year tour after OCS and zen buddhism suzuki some FT. Eustis transportation training before going to Nuremberg Germany with the 4th Armored Division and in my 2nd year I was the assistant S-3 to infant research, Col. George S Patton when the movie Patton came out. He was his son and later made General and I was able to talk to him about his dad for hours. He got invited to towns all over Europe because of the movie. After graduation ceremonies we all headed to separate officer basic schools. Mine was Ordinance Corps basic at Aberdeen Proving Grounds in MD. Mla Cover Paper. While many of our comrades in 515H were together through most of the infant papers Ft Dix, Ft Knox and Ft Belvoir route, only 6 of us were at Aberdeen. The others were Barnes, Hoch, Meeker, Moran and Oster. Officer Basic was pretty uneventful for me.

My first duty assignment was to Ft MacArthur located in San Pedro (the port of Los Angeles). After a couple of months running the motor pool the post commander announced that with my MBA I was the new Club Officer. Suzuki. That was an amazing experience. My new home was the top floor of a spacious duplex next door to the deputy post commander. My position meant I went on every trip on post army boat with the post commander: Mission to papers, clear the artillery range off the coast - translation = overnight fishing trip out and around Catalina Island. After OCS, I was assigned to the Armor Engineer Board at Ft.

Knox, KY where I tested equipment that could be purchased 'off the shelf' and then used in the field without having to go through the Army procurement process. While there I met and married my wife Donna Guidry Seales, who was the secretary to a basic training Brigade commander at Ft. Opinion In Austria. Knox. A month after we were married, in August of 1969, I went to infant papers, RVN where I was assigned to the 2nd CA Co and was attached to advisory team 90, based in Tay Ninh province on the Cambodian border northeast of Saigon. In addition to trying to opinion essay racism, win the hearts and minds of the local population through pacification projects, my main project was to build a 2 story concrete TB ward at the local hospital using local labor. Research. I was given $50,000 cash, plus all the cement and car research paper rebar I needed. I begged, borrowed and research papers 'liberated' everything else needed to build the building.

I also volunteered to fly with the Air Force FAC's attached to our team, where I had to watch VC doing 'close order' drill in their billets just across the border in Cambodia. I separated in Oakland in August of 1970, turning down a promotion with a guaranteed 11 months state side before they said I would get to return to RVN. While there I learned some people didn't like you if you had short hair and carried a duffel bag. I returned to work for the City of Los Angeles for essays zen buddhism suzuki a year before moving to Louisville, Kentucky where I worked for a design engineering firm a couple of years before going to work for a contractor in papers the area. In 1991 I went to work with the Kentucky Highway department from which I retired as an engineering supervisor five years ago. My wife of 43+years and I have three sons and two daughter-in-laws, who still live in the area, with two grandsons, 4 and 8, and a granddaughter 6, which our job is now is to bad grammar, spoil as much as possible. Bill Shugarts is a world class photographer who specializes in both military and missions photography. He has been published in various periodicals, newsletters, books, booklets, notecards, musical Cd covers, fund raising brochures/calendars and church/corporate publications and veteran's magazines.

Moreover, Bill currently leads a Military Outreach Ministry, is a docent for massage research the National Park Service at bad grammar the various Washington, DC memorials and a missionary with Christ United Methodist Church and an International PhotoMission Photographer. His photography was used in the United Methodist Church National Memorial Day broadcast and published in a successful fund raising brochure in the Virginia Dental Association publications. He is also a retired corporate executive who held key management positions with Westvaco, American Greetings, Reynolds/Alcoa including CEO responsibilities, has numerous local and global contacts and networks in military, missions business organizations. Bill has traveled extensively throughout the infant massage world in his 50 years of for college photography and is a decorated military officer from service in Vietnam with the Americal-23rd Infantry Division. Coming Full Circle 1st Lt. Robert T. Sprague. My fondest memory of OCS is the Mess Hall and all of the infant massage research yelling, questions, requesting to eat and trying to essays suzuki, get the first thumbnail sized piece of pie in my mouth before a booming voice from the infant massage research papers center of the zen buddhism suzuki room would say, CANDIDATES RISE, leaving that morsel hanging in mid air. That is why I would volunteer to clean the pot and pans. Infant. The sinks were out of sight, I was left alone and I had my choice of the pans that were filled with untouched bacon, cakes and other goodies. I proceeded to Aberdeen Proving Grounds for Ordinance training in vehicle repair and then was stationed at Fort Sheridan just outside of Chicago. One day I received an angry phone call to report to comparaticve essay outline, Col.

Meeker ASAP. Being new to the post I thought someone would know who and where the Col 's offices were. Who was the response. Papers. Panic . Shortly after and much to my chagrin, 515 Candidate Steve Meeker walked in the office. It was not too long afterward that I received my invitation to Vietnam. I was stationed just outside of Long Bin at a place called Bearcat as a Shop Officer of the 590th Maintenance Company and oversaw the repair of electronics, self propelled guns, jeeps, APCs, and trucks. I was the payroll officer and would drive to in austria, Vong Tau (50 miles away) with $30,000.00 in infant massage papers a paper bag (lots of cash in 1969), a side arm and a couple of guys with rifles. Outline. That's where I caught up with Bob Barnes in his billets listening to tapes like Hair. While over there I had a brief visit with Tom Dilatush and spent a couple of days with Dan Russell. It was great to infant massage research papers, see a few familiar faces and hear their stories. While other brave men were risking their lives in comparaticve essay the jungles, I had my own room with a stateside cherry bed and dresser and my own fan.

Food was hot, on massage research papers time, and the PX was nearby. I was merely a tourist for a year and on the sidelines as an observer. Returning to mla cover paper, the States in 1971 I was hired by Wyeth Laboratories and papers sold pharmaceuticals covering SE Ohio. I moved to Zanesville (50 mile East of Columbus), married Lynne Ann (42 years and counting), had two sons Josh and Sean, lived the same house for most of hybrid paper that time and retired in 2009 with 38 years with the same company. In reflection, I saw in those doctors office over the years many the massive health problems that others have faced changing their quality of life for the worse. I began thinking of infant research how fortunate I have been in my life, good family, education, 1st Lt, wonderfully married with two boys, and strived to be the best in my profession. That is mla cover research when it came to massage papers, me that after looking inward and concentrating on my family, trying to succeed, raising children and my own self interest , I had not really taken serious notice of most people's struggles and that they had not remotely tasted my success. Then I remembered the many unfulfilled hopes and dreams of the hybrid car research young names on infant massage the Vietnam Memorial. I decided that I could put my hard earned military training from Fort Belvoir to its best use. I am now volunteering to help prepare, serve and deliver 9,500 meals a month to mostly homebound people and essays suzuki the walk-in under privileged. I have re-found my original calling at the sinks washing pots and pans.

The only difference between OCS and Christ's Table is that all of the food is infant research used to help the needy and for college now and then I get a stray cookie, a slice of research papers cheese or a piece of melon. After OCS, I went to ordanance training at Aberdeen Proving Grounds until Feb. 1969. I then was assigned to opinion essay in austria, Ft. Infant Massage Research Papers. Dix to be maintenance officer at a maintenance facility for the Nike Hurcules missle sites in racism the NY/NJ area. I had orders to go to Viet Nam in September 1969 but just before I was going on leave before I was to report, Nixon started his pull back campaign and the division I was supposed to go to was the first to be brought home.

So my orders were changed and I stayed at Ft. Dix until I got out in Sept. 1970. I spent the infant massage last year as the hybrid post motor pool maintenance officer at Ft. Dix - a nice job.

I am a CPA and was with PricewaterhouseCoopers for 35 years after the army. I retired in 2004. My wife, Pam and I have 3 children, 3 grand-children and are expecting 2 more grandkids. Yes. I am delighted that you found me. I did not think I had any friends in that place. I remember that Peter Gagarin got me through the Math.(I could not balance my checkbook in those days, let alone classify a bridge.) I think the Tac Officers thought I was rich or politically connected. I was neither but, I think that's why I graduated.I spent an entire day calling a tree to attention because I had no command voice.

I almost quit halfway through but happily I was more afraid of my father than I was of the Tac officers. Infant Papers. A real Army Sergeant who was a member of comparaticve outline our class saved my butt when the Tac Officers made me the commander of the closing exorcises. He said, choose me as your Chief of Staff - I'll get everything done. You ride around in infant massage papers a jeep and call in % complete. That's what officers do. You'll be OK. I went to Germany, then to Viet Nam. Got a Bronze Star came home a Captain. I think I graduated last in our class.

I am still proud. Comparaticve Essay Outline. I went into the ministry in 1980. Infant Research Papers. But, On Memorial Day, Veterans Day September 11th, I am always asked to speak, not as Clergy, but as a Veteran as a neighbor. I wear the Bronze Star Pin in my Lapel every day. Mla Cover Page Research. If we have a reunion, I will do my best to infant massage papers, be there. Thank you for bad grammar essays finding me.

Rev Mike. I went to Basic Training at Fort Dix, NJ. Then, like a lot of us, I went to AIT at Ft Knox, KY. While at Ft Knox a buddy and infant massage papers I got sick of being Private E- nothings. We dressed up in civilian clothes, sneaked off the for college base and went to where I had stored my car (that I was not supposed to have) and drove to Louisville for an expensive dinner and infant papers time off base. On the way back, a Kentucky redneck in an old pick-up with no license and no insurance, crossed the Dixie Highway in front of bad grammar me. We crashed and infant research my beautiful 1965 Buick Riviera was almost totaled. The local police got there before the Military Police. They ticketed the senior essay other driver and had my car towed away. Papers. They explained to the MP's that except for secretly keeping a car off base and being AWOL, we were essentially innocent.

The MP's put us in their car and drove us ominously and wordlessly back to the base, shut their lights off at our barracks and stopped. One of mla cover paper them said: Get out. If you don't say anything, neither will we. A while later, before the car was repaired, I got orders to report to OCS. Infant Massage Research Papers. After I had been in OCS for bad grammar essays a few weeks, my parents went to Kentucky, got my car and drove to infant massage research papers, Fort Belvoir. Car Research. My father, who had been an Ordnance Officer in WWII and who could be very charming when he wanted to massage papers, be, stopped at the gate and asked the senior essay for college MP for an appointment with the Commanding Officer. Astonishingly, the massage papers Colonel agreed. The two of them got on well.

When my father told him about the incident with the car, the Colonel supposedly said: He can't have the car here either. Comparaticve Essay Outline. But, I have an idea. Let's put the car on the parade ground and let him look at infant papers it for six months. That should be punishment enough. So they did. Mla Cover Page Paper. Later on, when I was ready to quit the infant massage program, My father flew down in his small airplane, saw the car research Colonel again and asked for infant massage papers some time with me. The Colonel said: It's Easter Sunday, You can have one hour after church. By the time my father was through with me, I was no longer afraid of OCS.

My father was a Real Estate Broker in a small upstate New York city. He was not rich. Racism. But, I have often thought that the fact that he had his own airplane and seemed to be friends with the Colonel, is why the Tac Officers managed to get me through to graduation. They were mean enough to me, but I would have been too if I thought I was dealing with some spoiled rich kid with a high sense of entitlement. There was a Captain in papers our HQ who was an unhappy man. He had a job and could not seem to get it done.

He got nothing but grief from comparaticve essay outline, below and no support from above. So he gave up. Infant. He did nothing. Bad Grammar Essays. For reasons I do not remember, I was riding in a helicopter and research heard over the radio that that Captain had been relieved for non-performance of his duty. Comparaticve. I went through the infant massage papers list in my head and realized that I was the comparaticve essay outline only one in the HQ with that MOS. I knew that if I didn't do something quickly, I was about to suffer the massage papers same fate as that hapless Captain. I told the pilot to get me back to the compound right away. He did.

I ran up to the Colonel's office. He was a good man and had a West Point ring. I saluted and volunteered for essays suzuki the job under certain conditions. I wanted a helicopter and a pilot on call, a jeep of infant massage research my own (I didn't need a driver, even today I won't drive over a CocaCola can in the road) and two really excellent sergeants to work for me full time. I also wanted the Colonel to use his West Point clout whenever I could not get what I needed out of Saigon. The Colonel agreed. He then said: You are on a line so thin as to be practically invisible.

If you do this job, you'll get a medal. If you don't, you'll go to jail. Dismissed. A few hours later at supper, the Colonel approached me and as I stood up he whispered in my ear: You little SOB, you KNEW you were the only one here with that MOS. I keep my promises. Hybrid Car Research Paper. Take care of those boys.

I assumed responsibility for taking care of infant research 23 Mobile Advisory Teams scattered throughout the province. Bad Grammar Essays. The teams were comprised of two Lieutenants and three Sergeants based with a company of Vietnamese in little forts in the wilderness. The regs outlining what the five Americans were entitled to research papers, read like a Tolstoy novel. Suzuki. Generators, stoves, refridgerators, vehicles, fuel, ammunition, medical supplies, American food - the list was seemingly endless. But by the time I got there, the rotations were so precise that when one Team Leader got on the plane to go home, his replacement was landing.

For years, none of the teams knew what that were supposed to have. They were underfed (eating rice fishheads with the locals, underequipped and on research papers their own. I had met one of those Team Leaders at HQ. That's how I knew what I would need if I ever got stuck with that job. It gets better.

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How to Write A Cover Letter and A Follow-up Letter, and Create A Winning Portfolio. A cover letter is like the icing of massage research papers a cake or a movie poster. Basically, it aims to essays zen buddhism suzuki, tease potential employers and to give them an engaging reason to infant research, read through your resume. Remember that there are millions of job seekers out there, and to catch an employer’s eyes is a critical step in surviving a highly competitive field. The cover letter is not merely a summary or mirror of mla cover page research paper your resume, but an opportunity to build connection with your potential employer by infant massage personalizing the letter and revealing your style and tone in communicating. Aside from an attractive cover letter and a solid resume, applicants go through the process of waiting, which is car research paper quite a luxury nowadays for infant massage those who urgently need jobs.

Applicants can send follow-up letters to companies they are applying for, but they must do so with utmost courtesy. Essay For College! Sometimes, accompanying the cover letter and resume is a portfolio especially for industries that require heavy creative and technical work, like architecture, graphic design, and writing. This guide will look into infant massage research, the steps, techniques, and tricks in suzuki, coming up with an appealing yet professional: What is A Cover Letter. According to Yale College, a cover letter serves as a personal introduction to a prospective employer showing why you are interested in the position and the organization and justifying why you qualify for the position.

The letter should match what the job description is saying by emphasizing two or three strongest reasons why you are a good candidate for the role. How to Create An Engaging Cover Letter: The Characteristics. Length . Infant Research! The Harvard Law School website states that a cover letter should be one page only. It should have four to five paragraphs, with a font size of 11 or 12 and margins at 1-inch on all sides. Paper Quality and Size . The Adams Cover Letter Almanac stresses that the standard size of a cover letter should be 8 1/2-by-11 inch since other papers beyond this measurement might get lost in an employer’s file cabinet. The regular office papers may work for most positions, but executives and top-level positions are expected to deliver more expensive stationery papers, such as ivory laid. Tone . Accoridng to Harvard, the essays cover letter should be treated like a “one-sided interview,” like a conversation about massage research papers work with a colleague.

The tone of the cover letter remains professional but gives a glimpse of your individuality. It reveals how you speak or how you convey your desire or thoughts in a formal occasion. The Structure of A Typical Cover Letter. Address . Hybrid Car Research! The return address and massage research contact number at the top right-hand corner should appear without your name. It should also avoid abbreviations. The date can be placed two lines beneath the address and opinion racism in austria should not be abbreviated. Salutation . Using “To Whom It May Concern” is infant massage research not encouraged for page paper applicants.

One is advised by experts to find out the infant research papers name and position of the addressee, and confirm these details. Placed two lines beneath the address/date, the salutation can start with “Dear Mr.” or “Dear. Ms.” followed with the for college person’s last name and infant research a colon. For advertisements with no enough information and when all efforts to find out the zen buddhism suzuki name fail, you can use “Dear Hiring Manager,” “Dear Hiring Coordinator,” or simply “Good Morning.” First Paragraph . Based on the book Business Communication: In Person, In Print, Online by Amy Newman and Scot Ober, the opening paragraph must be straightforward as employers expect only research quality applications for the position.

Hence, it has to state: the reason for your letter the outline position you are applying for how you learned about the opening. The authors note that you can style your first few sentences based on your industry. For example, restrained expressions are for conservative industries, such as financial services, and something imaginative for creative industries like advertising and public relations. Take a look at massage papers the book’s example: For two years, I’ve been hoping Young and page research Rubicam would visit the Fullerton campus, so I was excited to massage research papers, see the essays suzuki summer position posting for Public Relations Assistant. From my PR coursework and PR experience, I’m confident that I have the strong written and oral communication skills you require for the position. As you can see, this is personalized but still professional and has all the necessary elements of a cover letter’s introduction. Body Paragraphs . The Harvard site claims that these paragraphs should establish the infant research papers work experiences that directly match the requirements the position is opinion racism requiring. It should explain “your background and includes why you are seeking work with this particular employer.” It has to infant massage research papers, clearly show why your skills meet the company’s needs, and essay outline make the connections very specific.

To deliver specific details, Newman and Ober wrote that an applicant can give an example of that connection. Such example should manifest modest confidence and not a sales approach. Here are some of their examples: Not : As stated on my resume, I sometimes went on sales calls. But : Once, I went on a sales call with the president of Scholastic, Inc.’s Education division, and we closed a $150,000 deal—the largest for infant research the Ugo software product. From observing the sales manager, I learned… Not : I took a class in page paper, business communication. But : The communication strategies I learned in my business communication class will help me resolve customer issues as a customer service representative at Allegheny Industries.

If you are to come up with two body paragraphs, the Cover Letter Alamanac suggests the following structure: Second Paragraph – Mention what you want to contribute to massage research, the company and how you can be an asset with your qualifications. You can also discuss your skills and how they go with the job requirements. Example: In addition to my strong background in mathematics, I also offer significant business experience, having worked in a data processing firm, a bookstore, and a restaurant. I am sure that my courses in statistics and computer programming would prove particularly useful in the position of Trainee. Third Paragraph – Explain why you choose the racism in austria company. You can touch on what you know about it (your research effort) and how familiar you are with the industry. Expressing your enthusiasm will help make your cover letter more appealing. Example: I am attracted to City Bank by your recent rapid growth and the superior reputation of your economic research department. After studying the papers different commercial banks, I have concluded that City Bank will be in car research paper, a strong competitive position to benefit from upcoming changes in the industry, such as the phasing out of Regulation Q.

In essence, after reading the massage research body paragraphs, the zen buddhism suzuki employer should realize what the applicant can bring to the position and to research papers, the company. Closing Paragraph . This is where you are supposed to thank the employer. Newman and Ober stress that the bad grammar last paragraph should mention the massage research papers applicant’s phone number and email address. The applicant is page paper expected to express flexibility in terms of schedule and infant papers location. Here are their examples:

After you have reviewed my qualifications, I would appreciate your letting me know when we can meet to discuss my employment with Connecticut Power and Light. I will be in essay for college, the Hartford area from December 16 through January 4 and could come to your office at any time that is convenient for massage papers you. When you feel like the company is senior essay for college receiving too many resumes, you can take a more proactive approach: I will call your office next week to see whether we can arrange a meeting to discuss my qualifications for the financial analyst position. As for massage research papers the closing line, one can use “sincerely.” Use powerful language. Avoid flooding your letter with too technical or jargon-heavy language to impress the hybrid paper employer. Infant Papers! This will be found in your resume and is useful when you become part of the company already. Page Paper! Right now, you are focused on showing your personality as an infant research papers, applicant and desirable employee. You can use action words to in austria, make the cover letter “alive.” Here are links to examples of those action words and phrases:

Do not mention your desired salary right away. Unless the infant massage research advertisement requires it, you can forget your concern about the salary. If it asks for an amount, give a range instead of an exact number. You can also note that it is negotiable. Avoid sounding informal and focus on essay for college being specific and concrete. Accoridng to the Adams Almanac, one should avoid comparisons or cliches like “I am a smart as a whip” or “Your company is known as the creme de la creme of massage research papers accounting firms.” Avoid using hot pink papers, sharing personal anecdotes such as how you found the advertisement while you were taking your dog for a walk, or talking about shortcomings and how desperate you are for essay outline the job. There is a proper venue for those personal details. Keep your cover letter focused on your qualifications and massage research papers the job’s requirements.

How to bad grammar, Compose Follow-up Letters. There are four types of follow-up correspondence according to massage research papers, the University of Washington: Thank-You Letter . This is given after a telephone conversation or face-to-face interview. The applicant should express appreciation about the time the company shared with him and the opportunity to further discuss his application. Here is a sample thank-you letter from Adams: Dear Ms. Cummings, It was a pleasure speaking with you today in connection with your search for a Department Manager. I feel my experience and achievements are well within the required qualifications set forth in your detailed job description, and I am confident of my ability to provide your department with the same dedication and capability as I exercise in my present position.

As outlined in my resume, I have extensive administrative and comparaticve essay fiscal management experience working with all departments on nonmedical aspects in a health care environment. The challenges associated with the above position are of great interest to me ad I would like the opportunity to research papers, meet with you, at your convenience, to further discuss my candidacy for this position. Declining Offer Letter . Sometimes, we are hesitant to say “no” to job offers. But we need to bad grammar, respond to them because in the future, your paths may cross and the networking aspect may be beneficial for both of you. Massage! Here’s an example from the Adams Alamanac: I received your letter dated May 8, offering me the Dental Hygienist position at Angle Dental Center. The compensation and benefits of the position sound very interesting, and I am confident I would enjoy working with your staff. However, shortly before receiving your letter, I was offered, and accepted, another position. Racism! Thank you for your time and consideration of my candidacy. Response to A Rejection Letter . Rejections happen because they are teaching you something or leading you closer to infant research, the work that is for comparaticve essay outline you.

To keep the connection and show sincerity, you can ask the company to consider you for future openings. Again, thank you for meeting with me last week to discuss the massage research opportunities for Architects at Edge Firm. I admire the innovative work you and your staff produce and, although I was not chosen for the position, I would like to zen buddhism suzuki, be considered for papers upcoming projects. I have enclosed an additional copy of my resume for your files. Thank you for your attension and bad grammar essays I hope to hear from infant research, you in the near future. Acceptance Letter . This kind of letter acknowledges and confirms the job offer (the tasks, the payment, and zen buddhism suzuki the schedule). This may also attest your solid work ethic. I am grateful for infant the opportunity to be part of such an esteemed department in the agency.

I look forward to mla cover page, assuming my post as Geographer, and I am happy to accept the terms of our agreement. As discussed, my graduation date is set for May 31, and I will be available to begin the infant research papers following Monday, June 13. I will contact your office early next week to mla cover research, schedule my training session. Again, thank you. I am very excited to begin. Here are other examples of cover and follow-up letters: Steps on infant massage research How to mla cover research, Create A Portfolio. Your career portfolio is the compilation of massage research your actual works.

This may be used by a potential employer to assess the essay in austria level of your talent, knowledge, and motivation. It is infant research actually a more visual “marketing tool” for your accomplishments and hybrid car research ability. This will give prospective companies ideas on the kind of work you can give them. The website of the Rivier University in New Hampshire lists the items you may include in your portfolio: Professional Growth and Career Planning. Outstanding work in your major Internship experience Professional memberships Job shadowing/Informational interviews Job descriptions/performance appraisals/work projects Professor evaluations College transcript. Academic or professional awards.

Communication Speeches/Oral Presentations Papers/written work Debates Articles written/published Business letters/correspondence. Sales/customer service experience Creativity Creative Writing Samples Art Work – drawing, painting, images Photography Theatre experience. Personal experience in another culture. Study of another culture Foreign Languages Innovative, imaginative or creative ideas Designing materials or projects. Critical Thinking and Research. Numeric work from coursework or professional experience Research papers/projects Case studies Care plans Critical analysis papers Critiques of research articles.

Leadership and Teamwork. Image source Clubs/Campus activities Athletics Academic group projects Leadership roles Community projects Volunteer activities Work or internship roles Active committee member or officer. Community outreach programs Fundraising events Community service events Multi-cultural awareness projects Campus ministry work Community building Care giving Service Learning. Technical and Scientific. Power Point presentations Excel spreadsheets Access databases Publisher (brochures, flyers) Computer hardware/software experience.

Web page design/programming Internet research Laboratory work Scientific reports Clinical skills. Extensive/Unique Travel Special Hobby Participation in a unique group Organizational memberships Licenses/Certifications Study Abroad Life Management Skills – time management, record keeping, managing, budgets or finances, etc. Resume, Letters, Credentials. Resume Letters of recommendation List of references Dean’s List letter Nomination letters Performance evaluation Letter of infant research papers acceptance into bad grammar essays, graduate school Letter commending your skills Image source. The Florida State University presents a list of specific materials you can add to infant papers, your portfolio: Statement of outline teaching philosophy (1-2 pages) Teaching honors/awards Evaluations (e.g., supervisor, student) Video of your teaching Evidence of student learning (e.g., graded exams, assignments [1 good/1 bad]) Classroom innovations (e.g., new technology) Sample lesson plans Relevant photographs. The Arts / Performance or Design. Actual work samples or photos of them Video/audio recording of work Course descriptions for infant classes/workshops List of competencies mastered. Here are links to sample portfolios that can serve as your guide: Bonus tips: As much as possible, your accomplishments should involve problems and then the solutions you formulated and suzuki executed alone or with a team.

Keep your portfolio neat and readable. Lastly, make sure that it will bring out your true style, the research one they can expect and help you grow.