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Great gatsby american dream term paper

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Great gatsby american dream term paper

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calculus bc homework AP Calculus BC Summer Homework (worth 200 points, approximately two full test grades) AP Calculus can help you earn university credit for Calculus 1 and, possibly, Calculus 2. Success requires a solid understanding of precalculus. This summer homework will ensure that you are well prepared to succeed, as well as facilitate a pace that will allow for plenty of time to practice for the AP exam near the end of the school year. This assignment is due on the first day of great american term school . Do not view this as extra work. Rather, by doing the online, summer homework you will be lightening your load during the school year and improving your chances to earn free university credit. Do not procrastinate. Start off the school year with an great gatsby paper A! Warning: If you don’t show clear work, or if it’s too messy to read, it will be returned to owl purdue apap, you to be redone. Please don’t make me struggle to read it or follow your reasoning. Concerns, questions, or discovery of great american dream term errors/typos in this document? Contact Beuschlein at . Google Classroom: Sign up now to receive information updates, assignments during the school year, etc. Use this code: uaf2f6.

Matrices: The required matrix problems below are part of a longer (but optional) matrix supplement that I’ve made. If you’re interested in doing the rest of the full supplement for optional credit, here’s the link . (The supplement doesn’t have to be done before the end of the ten steps, summer.) Theoretically, the matrix problems are review. Here is a link to a matrix presentation I made. Gatsby Term Paper? It addresses more linear algebra topics than you’re required to know, but feel free to use it as a reference. If you need a refresher on the basics of row ops, etc., there is no shortage of resources online. Solve each system by hand in three different ways: (i) using row ops on an augmented matrix, (ii) using an inverse matrix and matrix multiplication, and (iii) Cramer’s rule. Show all work (as always). a. 2x + 7y = -3 and -4x + 5y = 1. b. 3x + 4y -z = 0, x + 2y +3z = 18, and -2x + 2z = 12. Sorry, I realize that part (b) is zen buddhism, a pain in the hindquarters. I’m not trying to give you busy work. You’ll have to trust me that these procedures and great gatsby american dream term paper, operations are crucial to ten steps, understand, even though in practice you could solve the system in a jiffy with technology. (When you take linear algebra in college, or use matrices in your engineering or science courses, you’ll see why a good foundation is american dream term, so important.) In part (b) you may use technology to find the d a resume, inverse of the coefficient matrix, but in part (a) use the shortcut to get the inverse by hand.

Write this system as a single matrix equation in the form A v = b . The bold letters are vectors (matrices with a single column ). v has variable components of great gatsby american paper x, y, and z; b is college application help, comprised of three constants. A is gatsby american paper, called the coefficient matrix. Solve this system with a graphing calculator in the same three ways as above. Write down the syntax you use as well as some intermediate results (such as determinants). Just so you know, the fastest way to do this with a calculator is with the rref function. Owl Purdue Apap? Faster still would be to copy and paste the system into Wolfram Alpha and hit enter. a. f(g(?/8)) b. g(f(x)) c. f(g(x)) d. g(x) = -3/sqrt(2) e. g(x)/cos x = 1 (use double angle) f. g(x) = 5?/2 g. g(x) sec 2x / cot 2x. h. f(x + 1) i. f(2x - 1) j. f(x + h) k. [ f(x + h) - f(x) ] / h. a. Find y as a function of x. Great Term Paper? b. How To D A? What sort of trajectory does the particle follow? c. Where is the particle at t = 3? d. Where is it when its x-coordinate is 2? a. x = 5 cos t, y = 5 sin t b. x = 2 cos t, y = 3 sin t c. x = 2 sec t, y = 5 tan t. d. x = 3 csc t, y = 4 cot t e. x = 3 cos t, y = 2 sin t. b. Where they exist, locate the foci in each graph, and find the equations of any asymptotes. a. sine of a sum b. cosine of a sum c. Gatsby Dream Term? tangent of a sum. d. College Application? double angle for sine e. double angle for cosine f. double angle for gatsby dream term, tangent. g. power reduction for cosine h. Database And Dissertations? power reduction for sine i. circumference of a circle. j. Gatsby Paper? area of a circle k. total surface area of a cylinder l. College Application Help? volume of a cylinder. m. American Term? volume of college application essay help a cone n. volume of a sphere o. surface area of great american term paper a sphere. a. the surface area S of a cube in terms of its volume V. And Dissertations? b. the volume of a cone V in terms of its radius R, given that its height is three times it radius. c. the area of a walkway of width x surrounding a 50 ft ? 25 ft rectangular pool, in terms of gatsby american dream term paper x. d. the total surface area of a cylinder of height H whose diameter is how to d a resume, also H, in terms of H. a. a line with slope -3 through (2, 9) in slope-intercept form. b. a line that goes through (-4, -1) and great american term paper, (-2, 0) in point-slope form.

c. a line that makes a 45 ° angle relative to the x-axis with a x-intercept of -1 in standard form. a circle with center (-2, 1) and essays zen buddhism, radius 6. f. a parabola with vertex at great gatsby american dream paper, (3, 2) and focus at (3, 5). g. an ellipse inscribed in a box with vertices (-2, 1), (-2, 5), (6, 1), and (6, 5). Paper Apa? h. a circle that has endpoints of a diameter of (9,2) and (3, -8). i. a vertically-oriented hyperbola with asymptotes y - 2 = ±? (x - 3) and 10 units between its vertices. b. Confirm your answer to part (a) using synthetic division on and looking at great dream term, the remainder. m. where i is the imaginary unit (use Pascal). n. a. possible 5-card poker hands. b. the coefficient of x 3 y 7 in (x + y) 10. D A Resume? c. possible ways to great term, arrange the letters of MISSISSIPPI (it’s not 11! because of the repeat letters) d. 5 reds balls and 6 white balls are mixed in a sack. Five balls are chosen at random. What is the probability that at least three of essay writing them are red?

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Great Gatsby American Dream …

Lactoferrin receptor mediates apo- but not holo-lactoferrin internalization via clathrin-mediated endocytosis in trophoblasts. LfR [Lf (lactoferrin) receptor] is expressed in most mammalian tissues, including placental trophoblasts, and is presumed to mediate the internalization of Lf. However, the gatsby american term physiological significance of trophoblast LfR is not understood. Using the CT (cytotrophoblast) cell model BeWo, we demonstrated that transfection with LfR siRNA (small interfering RNA) significantly decreased apo- but not holo-Lf uptake compared with mock-transfected controls and that apo- but not holo-Lf significantly increased MMP (matrix metalloproteinase)-2 activity. As Lf functionality is related to the presence (holo-Lf) or absence (apo-Lf) of iron within the of african theses Lf molecule, our results suggest that apo-Lf may play a role in cellular invasion. Moreover, we detected LfR (?105 kDa) in great dream paper association with the plasma membrane, and ligand blotting confirmed that Lf binds to essay, a LfR of ?105 kDa. Apo-Lf treatment significantly increased LfR abundance at the plasma membrane and gatsby american dream paper, internalization probably occurs via clathrin-mediated endocytosis through early and recycling endosomes, as LfR was co-localized with EEA1 (early endosome antigen 1) and TfR (transferrin receptor) using confocal microscopy, and online college essay, hypertonic medium (0.4 M sucrose) significantly inhibited apo-Lf internalization. In summary, our data demonstrate that apo- but not holo-Lf is internalized by LfR and suggest that, following internalization via LfR, apo-Lf plays a role in CT invasiveness by gatsby american dream term inducing MMP-2 activity.

Moreover, LfR facilitates apo-Lf uptake specifically through clathrin-mediated endocytosis into early endosomes and potentially into a recycling pathway. Taken together, our data provide a new dimension in understanding ligand-dependant function that may be directly related to the ability of ten steps writing, LfR to selectively internalize apo- but not holo-Lf. BeWo cell endocytosis lactoferrin lactoferrin receptor matrix metalloproteinase-2 (MMP-2) The presence of a LfR [Lf (lactoferrin) receptor] was first identified in human small intestine [1]. Great Gatsby! It presumably facilitates the uptake of its protein ligand Lf, which is present in milk and mucosal secretions [2].

Therefore, kinetic studies on LfR have primarily been conducted in small intestine [3–6] and cultured enterocytes [7]. Of African Theses And Dissertations! We have previously cloned the LfR gene (GenBank® accession number NM_017625) [3] and gatsby american dream term, determined that the intestinal LfR is an ?110 kDa trimeric (?37 kDa subunits), glycosylated protein [7]. However, LfR is also expressed in non-intestinal tissues, such as heart, liver, spleen, thymus and skeletal muscle [3,8,9]. Recently, we detected LfR expression in mouse placenta associated with trophoblast cells [8], suggesting that LfR plays a role in this highly specialized cell type. Trophoblasts are divided into two subtypes; CTs (cytotrophoblasts) and owl purdue apap, STs (syncytiotrophoblasts), which are both maintained throughout pregnancy. The primary function of CTs is invasion into the uterine epithelium to secure successful implantation [10], whereas STs further implantation progression by establishing the nutrient and gas exchange barrier (maternal–fetal interface) and secreting hormones such as progesterone which are essential for dream paper, successful pregnancy maintenance [11]. The invasive capacity of CTs relies on the secretion of owl purdue apap, MMPs (matrix metalloproteinases), which function to degrade the surrounding ECM (extracellular matrix). Great American Dream Paper! Several disorders of pregnancy, including pre-eclampsia and spontaneous abortion, are believed to relate to ineffective CT proliferation, migration and invasion into of african and dissertations the uterine endothelium [10]. Lf has been attributed with multifunctional properties, including increased cell proliferation [12–16] and changes in cell morphology through activation of MMP transcription [17].

The pleuripotency of Lf may reflect the dramatic conformational change associated with iron binding and release [18,19] as holo-Lf (iron saturated Lf) and apo-Lf (iron free Lf) are known to adopt “closed” and “open” forms respectively [18,20]. For example, holo-Lf increases but apo-Lf decreases cell proliferation in human enterocytes (Caco-2 cells) [14] and while apo-Lf stimulates microvessel proliferation in rats, holo-Lf is ineffective [21]. Currently, the mechanisms through which apo- and holo-Lf exert their differential functions are unknown. Herein, we hypothesized that the great gatsby american dream term opposing functionality of apo- and holo-Lf reflects differences in cellular internalization mechanisms mediated via LfR. In order to address this, we utilized the human placental CT cell line BeWo and owl purdue apap, determined: (1) the effects of apo- and holo-Lf on MMP-2 activity, the major MMP in gatsby american paper trophoblasts; (2) the ability of the LfR to facilitate apo- and essays zen buddhism suzuki, holo-Lf uptake and great gatsby american term paper, internalization; and (3) the subsequent intracellular compartmentalization of LfR following Lf uptake. We also determined that apo- but not holo-Lf stimulates MMP-2 activity, that apo-Lf is internalized by LfR in a clathrin-mediated process and paper apa, that internalized LfR associates with the early endosome/recycling compartment. This suggests that functional differences between apo- and gatsby american dream term, holo-Lf are probably related to divergent uptake and intracellular trafficking mechanisms and provides a functional role for LfR in online college application trophoblasts. BeWo cells were purchased from the American Type Culture Collection (CCL-98, Rockville, MD).

Cells were maintained in gatsby dream paper complete medium containing F12 Nutrient Mixture (Ham) (Life Technologies), supplemented with 10% fetal bovine serum (Gemini Bio-Products), 1.5 g/l sodium bicarbonate, 10 ?g/ml streptomycin and 10 units/ml penicillin (Sigma) at 37 °C in online college essay a humidified atmosphere with 5% CO 2 until 90% confluent. For experiments, cells were sub-cultured at a ratio of 1:3 and great american dream term, cultured until confluent. Lf was isolated from human milk as previously described with slight modifications [22]. Essays Zen Buddhism! Casein was precipitated from great american dream term, pooled human milk with 0.06 M CaCl 2 , pH 4.6, for 1 h at room temperature (25 °C) with constant stirring and paper proposal apa, centrifuged at 8600 g for 20 min at 4 °C. The whey fraction was collected, centrifuged at great gatsby american dream term paper 8600 g for 20 min at research paper proposal 4 °C and then mixed with heparin–Sepharose Fast Flow resin (GE Health-Amersham Biosciences) overnight at 4 °C. The whey/resin mixture was centrifuged at 800 g for 5 min at 4 °C, the supernatant discarded and the remaining resin was packed on to a column and washed several times with wash buffer 1 (50 mM Tris/HCl, pH 8.0). To remove non-specific proteins, the column was rinsed with 5 bed volumes of elution buffer 1 (0.05 M Tris/HCl, 0.3 M NaCl, pH 8.0).

Next, Lf was eluted with 5 bed volumes of elution buffer 2 (0.05 M Tris/HCl, 1.0 M NaCl, pH 8.0) and fractions were collected. Lf-containing fractions were identified following Coomassie Brilliant Blue staining of SDS/PAGE (12%) gels. Samples were pooled and great gatsby dream paper, concentrated in YM-50 centrifugation tubes (Millipore Centriplus, Fisher Scientific) at how to d a resume 3000 g for gatsby term paper, 90 min at 4 °C. The protein concentration of purified Lf was determined spectrophotometrically ( A 280 ) as previously established [23]. Apo-Lf was prepared as previously described [24]. Holo-Lf was prepared with FeSO 4 (Lf/Fe, 1:2 molar ratio) in sodium bicarbonate (Fe/HCO 3 , 1:1 molar ratio) for 1 h at room temperature (25 °C). Unincorporated Fe was removed with YM-50 microcentrifuge tubes at 12000 g at 4 °C and stored at research ?20 °C. Fe saturation was determined spectrophotometrically by the A 460 / 280 ratio (22) [25].

To determine effects of apo- and holo-Lf on great american dream term paper, MMP-2 activity, cells were seeded in 24-well plates in complete medium until cells were 90–95% confluent. Cells were rinsed twice with 1?PBS and then incubated with apo- or holo-Lf (50 ?g/ml) in F12 medium plus 2% FBS (fetal bovine serum) for research apa, 4 h. Conditioned medium was collected after 24 h and concentrated to great gatsby american paper, one third of the original volume using YM-50 microcentrifugation tubes (Millipore Centriplus) at 5000 g for 20 min at zen buddhism suzuki 4 °C. Protein concentration of conditioned media was determined using the american dream Bradford assay (Bio-Rad) and proteins (20 ?g) were electrophoresed by how to resume SDS/PAGE (7.5% gel containing 1 mg/ml gelatin). Following electrophoresis, the gels were washed twice in 2.5% Triton X-100 in PBS for 15 min to remove SDS and great term paper, incubated in research proposal apa wash buffer 2 (50 mM Tris/HCl, pH 7.6, 10 mM NaCl, 50 mM CaCl 2 ) overnight at great gatsby dream term 37 °C. Gels were stained with Coomassie Brilliant Blue for 2 h and destained with 10% acetic acid and 40% methanol in online college application essay water. Absence of stain (representative of great gatsby dream term paper, proteolytic degradation) reflects MMP-2 enzymatic activity.

The relative densities of bands were quantified using the college application help Chemi-doc Gel Quantification System (BioRad). Apo- and holo-Lf were labelled with 125 I (GE Health-Amersham Biosciences) by the Iodogen method [26]. Great Gatsby American! Excess free iodine was removed with a PD-10 desalting column (GE Health-Amersham Biosciences). To determine Lf uptake, cells were cultured in 24-well dishes in growth medium until confluent. Cells were incubated with 125 I-labelled apo- or holo-Lf (10000–100000 c.p.m.) in SFM (serum-free medium) for 1 h at 37 °C. Medium was collected, cells were rinsed once with rinse buffer (0.5 M NaCl, pH 3.0) and pooled with the medium. Cells were washed three times with PBS, solubilized with 0.1 M NaOH and radioactivity was quantified in both medium and solubilized cells in a gamma counter. Affinity purification and validation of LfR antibody. LfR peptide (SSSPSLPRSCKEIKDE-Cys) was produced (Genemed Synthesis, South San Francisco, CA, U.S.A.) and conjugated (2 mg) to an affinity-purification column (Sulfolink purification kit; Pierce Biotechnology).

LfR antibody was purified from rabbit antiserum according to the manufacturer's instructions. How To D A! To demonstrate antibody specificity, affinity-purified LfR antibody (1 ?g/ml) was pre-incubated with LfR peptide (1 mg/ml) in 5% (w/v) non-fat milk in american term paper PBS-T (PBS, 0.1% Tween) for 2 h at room temperature (25 °C) prior to owl purdue apap, immunoblotting of cell lysate as described below. Ligand binding and immunoblotting. Confluent cells were lysed in homogenization buffer [20 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EGTA, 1?complete protease inhibitor (Roche Diagnostics)], sonicated three times for 10 s, centrifuged at great gatsby american term 3300 g for 15 min at 4 °C and the post-nuclear supernatant was collected. Protein concentration of the supernatant was determined by the Bradford method and college help, cell lysates (50–100 ?g protein) were resolved by electrophoresis. Gatsby American Paper! To determine Lf binding, cell proteins were resolved by non-reducing SDS/PAGE at 80 V for of african, 4 h and great american term, transferred on to nitrocellulose membrane. Online College Application Essay! Membranes were incubated with 5% BSA in PBS-T for 1 h at paper room temperature (25 °C) to block non-specific binding. Membranes were incubated overnight with 125 I-labelled apo-Lf, blots were rinsed twice with PBS-T and bound ligand was visualized by autoradiography. For immunoblotting, cell extracts were elecrophoresed by resume SDS/PAGE (10% gel, 200 V for 1 h) under reducing conditions and membranes were incubated with LfR antibody for 1 h. Membranes were washed in PBS-T and primary antibody was detected with donkey anti-rabbit IgG-HRP (IgG-horseradish peroxidase; GE Health-Amersham Biosciences) in 5% (w/v) nonfat milk in PBS-T.

Membranes were washed in great dream PBS-T for 30 min, visualized with Super Femto Detection Chemiluminescence Reagent (Pierce Biotechnology) and exposed to how to, autoradiography film. Gatsby American Paper! Relative band density was quantified using the owl purdue apap Chemi-doc Gel Quantification System (Bio-Rad). Transient transfection with LfR siRNA (small interfering RNA) Cells were seeded on to 24-well or 6-well plates and american, cultured until 50% confluent, then transfected with 80 pmol of LfR siRNA (Ambion) in Lipofectamine™ 2000 (Invitrogen) for 24 h according to paper, the manufacturer's instructions. Cells treated with Lipofectamine™ 2000 alone were used as mock-transfected controls. Great Gatsby American! After 24 h, cells were incubated with 125 I-labelled apo- or holo-Lf in SFM at online college help 37 °C. Lf uptake was measured after 30 min as described above. Great American Term! To verify LfR suppression, transfected cells were scraped into 25 ?l of homogenization buffer (20 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EGTA, 1?complete protease inhibitor) and immunoblotted as described above. Biotin-labelling of d a resume, cell surface proteins.

To determine LfR abundance at the plasma membrane, cells were seeded on great american dream term, to 100-mm-diameter dishes and cultured until confluent. Cells were rinsed with PBS and the cell surface was biotinylated with sulfo- N -hydroxysuccinimido-biotin (0.5 mg/ml; EZ-Link Sulfo-NHSS-Biotin; Pierce) at owl purdue apap 4 °C for 1 h. Cells were rinsed with ice-cold 0.2 M glycine buffer, pH 5.0, and then with cold PBS to remove unbound biotin. Cells were lysed with lysis buffer [50 mM Tris/HCl, pH 7.4, 2 mM EDTA, 2 mM EGTA, 1?protease inhibitor (Roche Diagnostics)], sonicated (three 10 s bursts) and centrifuged at 100000 g for great gatsby american dream term, 20 min at 4 °C. The supernatant was discarded and the pellet was resuspended in lysis buffer with 0.2% SDS and incubated at database theses and dissertations 60 °C for 5 min. As the samples cooled, 1% Triton X-100 was added, the samples were re-sonicated and centrifuged at 100000 g for 20 min at 4 °C to pellet any insoluble material. The supernatant was collected and incubated with 300 ?l of slurry (1:1) of immobilized avidin beads (UltraLink Immunobilized Monomeric Avidin; Pierce) for 1 h at room temperature (25 °C) with constant rotation. To collect proteins bound to the resin, the samples were centrifuged at 2300 g for 1 min. The supernatant was discarded and the beads were rinsed with PBS and great gatsby paper, centrifuged for 1 min at research paper 2300 g . To elute biotinylated proteins, an great american dream paper equal volume of of african and dissertations, Laemmli sample buffer (Bio-Rad) containing 2-mercaptoethanol (5%) was added and incubated at 90 °C for 5 min.

The samples were centrifuged at american paper 2300 g for 1 min and biotinylated proteins were resolved by SDS/PAGE and immunoblotted for LfR as described above. Determination of research apa, GPI (glycosylphosphatidylinositol)-anchoring by great gatsby american dream Pi-PLC (phosphatidylinositol-specific phospholipase C) cleavage. To determine if LfR is essays zen buddhism a GPI-anchored protein, cells were seeded on great american paper, to 24-well plates and pretreated with Pi-PLC (1 unit/ml; Invitrogen) in SFM for 1 h at 37 °C to cleave GPI-anchored proteins. To verify the ten steps effects of dream term paper, Pi-PLC on database and dissertations, LfR abundance at the plasma membrane, cell surface proteins were biotinylated and immunoblotted for LfR as described above. To determine the effects of Pi-PLC on Lf uptake, cells were rinsed with PBS, fresh SFM was added and cells were incubated with 125 I-labelled apo-Lf at 37 °C and uptake was measured after 1 h as described above. To illustrate the efficacy of the enzyme, spent medium was collected and concentrated to one third of the gatsby original volume using YM-50 microcentrifugation tubes (Millipore Centriplus) at 5000 g for 20 min at essays suzuki 4 °C and immunoblotted for ALP (alkaline phosphatase), a known GPI-anchored protein. To inhibit endocytosis, cells were seeded on to 24-well plates and treated with hypertonic medium (0.4 M sucrose). Cells were pre-treated in hypertonic medium for american dream, 30 min and incubated with 125 I-labelled apo-Lf in essays SFM at american dream paper 37 °C in the presence of 0.4 M sucrose and essay, uptake was measured after 60 min. Lf uptake was measured as described above.

To determine if de novo LfR protein synthesis is required for Lf uptake, cells were seeded on to 12- or 24-well plates until confluent and pretreated with cycloheximide (Cyhex, 10 ?g/ml, Sigma) in gatsby american dream paper SFM for 6 h at 37 °C. Cells were either collected for SDS/PAGE analysis or incubated with 125 I-labelled apo-Lf in owl purdue apap the presence of great american dream term, Cyhex at 37 °C and Lf uptake was measured over 7 h. To determine the subcellular localization of LfR, cells were seeded on to glass coverslips, cultured for 3–4 days in growth medium and incubated with apo-Lf (where indicated, 200 ?g/ml) in SFM for up to 1 h at database theses 37 °C. After treatment, cells were rinsed with PBS and fixed with 4% phosphate-buffered paraformaldehyde for 10 min and gatsby, permeabilized with 0.2% Triton X-100 in PBS for 5 min. To eliminate non-specific binding, cells were incubated in blocking buffer (5% goat serum in PBS) for 30 min followed by and dissertations incubation with affinity-purified LfR antibody (0.8 ?g/ml) in gatsby term paper PBS containing 5% goat serum, 1% BSA and 0.2% Triton X-100 for 1 h. Cells were rinsed with PBS and LfR was detected with Alexa 488-conjugated-anti-rabbit IgG (1 ?g/ml; Molecular Probes) in PBS containing 0.2% Triton X-100 and 1% BSA for 20 min. Paper Apa! Co-localization of LfR with specific subcellular markers was determined following incubation with antibodies for clathrin (1:500, Abcam); EEA1 (early endosome antigen 1; 1:500, Abcam), TfR (transferrin receptor, localizes to recycling endosomes, 1 ?g/ml; Zymed Laboratories); LAMP1 (lysosome-associated membrane protein 1; 1:200, Abcam); M6PR (mannose 6-phosphate receptor, localizes to late endosomes; 1:500, Abcam) and gatsby term paper, detected with Alexa 568-conjugated-anti-mouse IgG (1 ?g/ml; Molecular Probes). After several rinses, coverslips were mounted with ProLong Gold (Molecular Probes) and sealed with nail polish. Immunofluorescence imaging was performed using an Olympus BX50WI microscope with UPlanApo ?100 magnification under an how to resume oil-immersion lens [NA (numerical aperture), 1.00], and digital images were captured using the Bio-Rad Radiance 2100 confocal system with LaserSharp2000 software, version 4.1 (Bio-Rad). Results are presented as means±S.D. of great dream term paper, triplicate samples from 2–3 separate experiments. Statistical comparisons were performed using Student's t test or one-way ANOVA (Prism Graph Pad) and significance was set at P 0.05.

Active form of MMP-2 is increased by essays apo- but not holo-Lf. Secretion of MMP-2 plays a key role in CT invasion, thus we determined effects of Lf and Lf conformation on MMP-2 activity in BeWo cells. Using gelatin zymography, our data demonstrated that apo-Lf significantly increased (20%) MMP-2 activity compared to untreated cells (Figure 1). Great Gatsby American Term Paper! In contrast, we observed no effect of holo-Lf on MMP-2 activity, suggesting a differential effect of Lf conformation on college application, cellular invasivity of CTs. Representative zymogram (upper panel) of culture medium collected from cells treated with either apo- or holo-Lf. Equal amounts of protein (20 ?g) were electrophoresed through polyacrylamide gels containing gelatin and stained with Coomassie blue. MMP-2 activity was detected as clear bands (digested gelatin) on a blue background.

MMP-2 activity was significantly increased in cells treated with apo-Lf as compared with untreated (control) and holo-Lf treated cells (lower panel). Data shown represent MMP-2 activity as a percentage of untreated control cells (mean±S.D.) for two independent experiments ( n =4). * P 0.05. LfR facilitates uptake of apo- but not holo-Lf. To determine if LfR mediates apo- and/or holo-Lf uptake, uptake studies using 125 I-labelled Lf were performed. Our data demonstrated that BeWo cells internalized 9-fold more apo-Lf compared with holo-Lf (Figure 2). Great Gatsby! Using affinity-purified LfR antibody, we confirmed that BeWo cells expressed LfR, as specifically immunoreactive proteins were detected at ?35 and ?105 kDa in BeWo cells (Figure 3). Of African Theses! Ligand binding plays a critical role in receptor function, and ligand binding established that radiolabelled apo-Lf bound to two LfR proteins at great term ?75 and ?105 kDa (Figure 3), but not to owl purdue apap, the 35 kDa protein. We speculate that the 35, 75 and 105 kDa moieties represent LfR monomers, homo-dimers and great american term, -trimers, respectively. The ability of LfR to internalize apo-Lf is dependent on its cellular localization and we showed that the 105 kDa LfR was localized to the plasma membrane using cell surface biotinylation followed by immunoblotting (Figure 4A). Of African Theses And Dissertations! In contrast to what was reported for the intestinal LfR [3], trophoblast LfR does not appear to great term paper, be GPI-anchored as demonstrated by owl purdue apap internalization of Lf following GPI-anchored protein cleavage with Pi-PLC (Figure 4B).

Confirmation of Pi-PLC cleavage was verified as Pi-PLC increased ALP abundance in spent medium compared to untreated cells (Figure 4C). Confluent cells were treated with 125 I-labelled apo- or 125 I-labelled holo-Lf and Lf uptake was determined. Gatsby American Term Paper! Representative graph of uptake after 1 h illustrates that significantly less holo-Lf was internalized when compared to apo-Lf. Online Application Help! Data shown represent mean Lf uptake (Lf uptake/mg protein)±S.D. from two independent experiments ( n =4). Great Term! * P 0.001. Representative immunoblot ( A and B ) and ligand blot ( C ) of cell lysate proteins. Research Paper Proposal Apa! Proteins (50 ?g) were resolved by great gatsby american dream term paper SDS/PAGE and immunoblotted with LfR antibody ( A ; LfR Ab, 1 ?g/ml) or pre-absorbed with excess Lf antigen ( B ; LfR Ab+peptide) for writing, 1 h. Great American Term Paper! The LfR antibody detected specific proteins at ?105 and ?35 kDa ( A ) which were both completely eliminated following pre-absorption with LfR peptide ( B ). ( C ) Cell lysate (100 ?g) was resolved under non-reducing and non-denaturing conditions, and incubated with 125 I-labelled apo-Lf. Detection of ligand binding to proteins at 105 and zen buddhism suzuki, 75 kDa was observed. Confluent cells were treated with Pi-PLC and surface expression and term, Lf uptake was assessed. ( A ) A representative LfR immunoblot of biotinylated cell surface proteins documents the presence of LfR on d a, the plasma membrane after Pi-PLC treatment. Dream Term! ( B ) Cleavage of GPI-anchored proteins with Pi-PLC did not significantly reduce 125 I-labelled apo-Lf uptake in application essay BeWo cells.

Data represent mean Lf uptake/mg protein±S.D. for two independent experiments ( n =4). Dream Term Paper! ( C ) Representative immunoblot of ALP antibody from total spent medium following Pi-PLC treatment, validating enzyme activity. In order to determine a role for LfR in Lf uptake, LfR expression was attenuated in cells transfected with LfR siRNA (Figure 5A). We demonstrated that reduced LfR abundance resulted in significantly lower apo-Lf uptake (30%) compared with mock-transfected controls (Figure 5B). In contrast, holo-Lf uptake was not significantly reduced (10%), suggesting that internalization of paper, holo-Lf in BeWo cells is not predominantly LfR-mediated. Great Gatsby American! Due to our finding of limited LfR-mediated holo-Lf uptake and resume, lack of an great gatsby paper effect of holo-Lf on MMP-2 activity, we subsequently focused on effects of apo-Lf on the intracellular trafficking of LfR. ( A ) Representative LfR immunoblot of owl purdue apap, total protein illustrating significant attenuation of LfR compared to dream term paper, mock-transfected cells. ( B ) Lf uptake was measured using 125 I-labelled apo- or 125 I-labelled holo-Lf in owl purdue apap cells transfected with LfR siRNA or mock-transfected controls. Data represent the means±S.D. Great American Dream Term Paper! ( n =2–3) of triplicate experiments. * P 0.05, ** P 0.01, as analysed by Student's t test. LfR internalization is a clathrin-dependent process and potentially follows a recycling pattern. As our data clearly demonstrated that apo-Lf is internalized via LfR, we then determined the mechanisms responsible for owl purdue apap, internalization of apo-Lf via LfR.

CTs were treated with apo-Lf and LfR protein abundance on the plasma membrane was assessed following cell surface biotinylation. We noted that treatment with apo-Lf resulted in a rapid (10 min) and significant increase in cell surface-associated LfR (Figure 6A), independent of changes in total LfR abundance (Figure 6B). This suggests that apo-Lf may signal the recruitment of LfR to the plasma membrane or that perhaps Lf binding to LfR transiently interferes with its internalization. Confluent cells were treated with or without apo-Lf and LfR surface expression was assessed. ( A ) Representative immunoblot of crude membrane proteins from cells treated with apo-Lf or untreated and detected with LfR antibody. Representative graph illustrates that LfR abundance significantly increased with apo-Lf treatment compared to great gatsby american dream term, untreated cells with no effect on essay writing, the total amount of LfR protein expression ( B ). * P 0.001, as analysed by american Student's t test. College Help! ?-actin was used as a loading control. Data represent means±S.D. Great Gatsby Term! ( n =3) of 3 independent experiments. Multiple pathways for internalization of paper proposal apa, plasma membrane proteins have been characterized. American! To identify the mechanism responsible for LfR internalization, CTs were treated with hypertonic medium (0.4 M sucrose) to inhibit clathrin-dependent endocytosis, which resulted in a robust inhibition of apo-Lf internalization (40%) compared with untreated cells, with values of 2.4±0.25 and theses, 5.7±0.42 pmol/mg protein±S.D. for treated and great gatsby american paper, control cells respectively; n=5–8 per group, * P 0.001. To confirm the owl purdue apap mechanism by which LfR is endocytosed, we used confocal microscopy to identify the subcellular compartments within which LfR resides. Great American Term Paper! As indicated in Figures 7(A) and 7(B), LfR and clathrin co-localized and were largely associated with the plasma membrane, as illustrated by yellow pixilation in paper apa the merged images (Figure 7C).

As internalization is ligand-stimulated, cells were treated with apo-Lf, and indirect immunofluorescence was used to great gatsby term, visualize specific sub-cellular compartments within which LfR was associated. Following ligand stimulation, we demonstrated partial co-localization of owl purdue apap, LfR and the early endosome marker EEA1 in vesicular/endosomal-like structures (Figures 7D–7F). As co-localization between EEA1 and LfR was not exclusive, we sought to great gatsby paper, examine other sub-cellular compartments containing LfR. Of African Theses! Clathrin-mediated endocytosis is one mechanism cells utilize to control cell surface receptor abundance and turnover via recycling. To investigate whether LfR trafficking from the plasma membrane potentially includes endocytic recycling, we used the indirect immunofluorescence of TfR as a marker for recycling endosomes. We showed partial co-localization of LfR and TfR within distinct internalized vesicles in cells following ligand stimulation (Figures 7G–7I).

No co-localization between LfR and M6PR (a late endosome marker) and minimal co-localization with LAMP1 (a lysosome marker) was detected (results not shown). These results indicate that, in response to apo-Lf stimulation, LfR is internalized via clathrin-mediated endocytosis and consequently enters a distinct intracellular endocytotic pathway. Confocal microscopy identifying specific sub-cellular compartments associated with LfR. Cells were fixed with paraformaldehyde and imaged for LfR (green) and sub-cellular markers (red); clathrin ( A and C ), EEA1 ( D and F ) and TfR ( G and I ). Clathrin ( A ) and great american term, LfR ( B ) were co-localized (yellow, C ) and college help, stained in a plasma membrane-like pattern in cells. Gatsby American Dream! Following apo-Lf treatment, partial co-localization with the early endosomal marker EEA1 ( D ) and LfR ( E ) was detected (yellow, F ) and treatment of cells with apo-Lf clearly resulted in vesicularization of LfR.

Furthermore, co-localization with the recycling endosome marker TfR ( G ) and LfR ( H ) was detected (yellow, I ) following treatment of cells with apo-Lf. Finally, to determine if increased abundance of LfR at research paper proposal apa the plasma membrane in response to apo-Lf stimulation reflects changes in intracellular LfR recycling, we blocked de novo protein synthesis with Cyhex and measured apo-Lf uptake. Gatsby American Paper! Inhibition of new receptor synthesis did not diminish apo-Lf uptake, indicating that LfR is recycled. In fact, apo-Lf uptake was significantly higher in research paper proposal apa Cyhex treated cells (Figure 8A), suggesting that apo-Lf enhances LfR recycling. Moreover, our data indicated that CT-derived LfR is a relatively long-lived protein (Figure 8B), as abundant LfR remained detectable after 7 h of Cyhex treatment and that de novo protein synthesis may play a minor role in sustaining total and great gatsby term paper, surface receptor abundance. Confluent cells were pre-treated with Cyhex and of african, uptake of 125 I-labelled apo-Lf and american term paper, LfR abundance was assessed compared with untreated cells. ( A ) Apo-Lf uptake from cells significantly increased in the presence of cycloheximide (Lf+Cyhex) after 3 h compared with untreated cells (Lf). Research Paper! Data represent means±S.D. for two independent experiments ( n =3–4), and * P 0.05 compared with untreated cells, as analysed by Student's t test. ( B ) Representative immunoblot of total protein extract isolated from cells pre-treated with Cyhex then incubated with apo-Lf for 3 and 7 h compared with untreated cells. The immunoblot demonstrates that Cyhex did not affect LfR abundance. ?-Actin was used as a loading control. CTs are specialized cells in the placenta that are initially responsible for american dream term, invasion into owl purdue apap the uterine endothelium, assuring optimal fetal development [10]. Our previous detection of LfR along the great gatsby dream maternal and fetal interface in the placenta implicates LfR in how to CT function [8,9,27].

The aim of the present study was to american paper, explore the function of Lf in the placenta as mediated via LfRs using a CT cell model. While primarily regarded as a component of milk [2], Lf is also found in decidua (9–95 ?g/g), amniotic fluid (2–37 ?g/g) and database and dissertations, chorion (2–26 ?g/g) [28], and is secreted directly from uterine epithelia [29,30]. This suggests that Lf secreted from the uterine endothelium may regulate CT function in a paracrine manner. Results from this study documented an enhancing effect of apo- but not holo-Lf on MMP-2 activity in CTs, suggesting a regulatory role for great gatsby american term paper, apo-Lf in CT invasivity as this process is online college application essay dependent upon the secretion and activation of MMPs. There is precedence for effects of great gatsby american dream term paper, Lf on research paper proposal apa, MMP regulation as over-expression of great gatsby american dream paper, Lf activates MMP-1 expression through binding to AP-1 promoter elements and through stimulation of the p38 MAPK (mitogen activated protein kinase)-dependant pathway in mouse fibroblasts and human COS-1 cells [17]. We speculate that apo-Lf may similarly regulate the transcription of ten steps essay writing, MMP-2 via AP-1 promoter elements which are present in the MMP-2 gene [31]. In support of the concept of Lf acting as a transcription factor, a nuclear localization signal within the N-terminal region of the Lf molecule (amino acids 1–5) has been previously identified [32], and Lf binds to gatsby term paper, DNA [33,34]. Alternatively, the induction of of african and dissertations, MMP-2 via apo-Lf could be through the p38 MAPK pathway as p38 activation is great gatsby american paper required for the up-regulation of MMP-2 protein expression [31]. Owl Purdue Apap! The ability of Lf to great dream term paper, activate p38 MAPK has been previously shown in other cell types [16,35], providing support for a direct role of apo-Lf on CT invasivity.

The effect of apo- but not holo-Lf on MMP-2 activity probably reflects that distinct functions are associated with either apo- or holo-Lf. Research Paper Proposal! In contrast to reports in rat hepatocytes [36], CTs bound and american dream, internalized both apo- and holo-Lf. How To D A! However, in CTs, apo-Lf was internalized more avidly than holo-Lf, suggesting preferential uptake mechanisms specific to apo-Lf which may reflect the requirement of gatsby term, this molecule for CT-specific functions such as invasivity, while precise Lf-dependant functions in hepatocytes remain unknown. Gene attenuation of LfR conclusively determined that LfR primarily facilitates apo-Lf uptake in CTs. Recently, distinct Arg-Gly-Asp (RGD) binding motifs were found within the Lf molecule [37], which have been postulated to determine ligand binding and receptor interactions.

It is resume possible that iron-saturation, which alters Lf conformation, may result in LfR inaccessibility to these binding motifs. While this is not to imply that holo-Lf binding and great gatsby american dream, uptake does not elicit cellular events, it does not appear that these effects are mediated via LfR internalization in CTs. Possibly, holo-Lf internalization is not a prerequisite to stimulate cellular events at the plasma membrane as suggested by other investigators [15,35]. As apo-Lf was preferentially taken up by LfR, we sought to help, characterize the placental LfR more extensively. Great Gatsby American Dream Term! It has previously been reported that intestinal LfR is a GPI-anchored protein [3]. Contrary to this observation, we determined that, in CTs, the LfR receptor is owl purdue apap not a GPI-anchored protein and is in great gatsby american term paper fact internalized via clathrin-mediated endocytosis following ligand binding. Our observations in online trophoblasts are consistent with previous reports in american dream term other cell types such as hepatocytes, where inhibition of clathrin-mediated endocytosis by hypertonic medium [38] significantly reduced Lf internalization.

We speculate that differences in Lf uptake between intestinal and CT LfR may reflect differences in cellular function, as CTs are a semi-pleuripotent and essay writing, invasive cell type, whereas enterocytes are terminally differentiated polarized cells involved in vectorial nutrient transport. Our determination that LfR is internalized via a clathrin-dependent mechanism may provide insight into LfR structure, as receptors utilizing clathrin-mediated endocytosis require a cytoplasmic, tyrosine-based sorting signal for gatsby american dream term, interaction with adaptor proteins, such as AP2 [39]. To identify potential AP2-interacting motifs within the LfR sequence we used a proteomics server ( and indeed two tyrosine-sorting motifs were identified at ten steps amino acids 120–123 and great american dream, 129–142. This further suggests that a portion of LfR protein does reside intracellularly and that one or more of these motifs may functionally interact with AP2 to database of african theses and dissertations, facilitate LfR internalization. LfRs have been identified in various cell-types and gatsby, reports have determined a molecular mass of of african and dissertations, ?110 kDa [8]. Recently, Tsuji et al. [40] examined the gatsby american dream term structure of human LfR in greater detail and determined that it is an 105.5 kDa oligomeric protein made up of research paper proposal, three 35.5 kDa subunits, similar to what we observed in trophoblasts. Interestingly, while we detected both the monomeric (35 kDa) and trimeric (105 kDa) forms of LfR under reducing conditions, the great american dream term paper abundance of the trimer was always greater that the monomer.

For some reason, we were unable to extensively reduce the trimeric form to monomers even when using dithiothreitol and iodoacetic acid (V. Lopez, unpublished observations). Interestingly, we did not detect the dimeric form of LfR under reducing conditions, which is similar to the findings of ten steps essay writing, Tsuji et al. [40]. We further demonstrated that apo-Lf binds to both dimeric and trimeric LfR multimers, which agrees with previously published reports demonstrating that human LfR forms a homo-trimer [7,40] as a result of subunit oligomerization [9]. We speculate that the trimeric form is an unusually stabilized form, mediated by gatsby dream term paper several intracellular disulfide bonds and possibly also hydrophobic interactions, whereas the dimeric form is less stable and proposal, can be reduced to the monomeric form. In contrast, a 45 kDa calcium-dependent LfR has been reported in rat hepatocytes [41]; however, it is gatsby american unknown how this LfR relates specifically to Lf function as rats do not express Lf. It is well established that cell signalling and cellular homoeostasis are altered by increasing or decreasing cell surface receptor abundance [42]. As LfR abundance at the plasma membrane did not require de novo synthesis, our data suggest that recycling of LfR to and from the plasma membrane occurs. Endocytic recycling requires internalization of cell surface receptors and their ligands within clathrin coated pits [43], with the zen buddhism interaction between transferrin and TfR being the classic example of this process [44]. Receptor recycling is one strategy by which cells maintain a mobilizable receptor pool to quickly traffic to the cell surface in response to physiological stimuli, to allow cells to redistribute cell-surface molecules to great term, specific cell locations or compartments undergoing a physiological activity [45].

We speculate that Lf may either stimulate the recruitment of LfR to the plasma membrane, or that perhaps Lf binding to owl purdue apap, the receptor results in great dream term paper transient accumulation of suzuki, LfR prior to internalization via clathrin-mediated mechanisms. We propose that CTs may require Lf/LfR internalization to american term, rapidly facilitate implantation, allowing for optimal embryo-fetal development. Further studies are needed to writing, address this issue. Collectively, the great results from this study demonstrated a specific role for apo-Lf but not holo-Lf in regulating MMP-2 activity and owl purdue apap, thus potentially endothelial invasion in CTs. Importantly, we have determined that apo- but not holo-Lf is great gatsby internalized via LfR, which occurs through a clathrin-dependent pathway in how to resume CTs suggesting a mechanistic and gatsby dream paper, functional difference between apo- and holo-Lf. These results may help to explain the pleuripotent and application, cell-specific functions which have been associated with Lf.

The authors would like to express their gratitude to Dr. Lisa L. Gough for dream term paper, critical reading of the manuscript. Proposal! This work was supported by NIH grant number HD-043240. Abbreviations: ALP, alkaline phosphatase; apo-Lf, iron free lactoferrin; CT, cytotrophoblast; Cyhex, cycloheximide; EEA, early endosomal antigen; GPI, glycosylphosphatidylinositol; holo-Lf, iron saturated lactoferrin; LAMP, lysosome-associated membrane protein; Lf, lactoferrin; LfR, Lf receptor; M6PR, mannose 6-phosphate receptor; MAPK, mitogen activated protein kinase; MMP, matrix metalloproteinase; Pi-PLC, phosphatidylinositol-specific phospholipase C; siRNA, small interfering RNA; SFM, serum-free medium; ST, syncytiotrophoblast; TfR, transferrin receptor.

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Andre Bazin Auteur Theory Essays and Research Papers. Great Gatsby American Dream Paper! Translated from the database theses French word author, auteur theory was first introduced in the French film journal Cahiers du Cinema. The . theory holds that a director is the primary person responsible for the creation of a motion picture and instills it with his or her distinctive style. It is a medium for the personal artistic expression of the director. In order for a director to be considered an auteur , there must be a consistency of gatsby dream paper style and theme across a number of essays zen buddhism suzuki films.

The film theoretician and founder. Gatsby Paper! Alfred Hitchcock , Auteur theory , Darren Aronofsky 1686 Words | 5 Pages. Window vs. Frame? Sergei Eisenstein: The Formalist Andre Bazin : The Realist The language of cinema has been developed by . trail and error over the past one hundred years by pioneers venturing into uncharted territory. Today anyone with a T.V. set is accustomed to suzuki the language, perhaps without even realizing they are taking part in a “conversation”.

This conversation has to be created by using a cinematic language. Among such pioneers that crafted this language are, Sergei Eisenstein, a man who. Andre Bazin , Dialectic , Film 1230 Words | 4 Pages. “… As far as I know, there is no definition of the auteur theory in the English language, that is, by an American or British . American Term! critic” (Sarris 1962) was the opening line to Andrew Sarris’s famous “Notes on the Auteur Theory in 1962” essay. D A! This essay is what brought the “ auteur theory ” in to the spotlight in gatsby term paper, the USA. And to ten steps essay writing today, this theory is still in hot debate. Trying to gatsby paper figure out whether or not the director is the lone “ auteur ” of theses and dissertations a film is american term paper a tough claim to make. In an article for Slate Magazine. Alfred Hitchcock , Andre Bazin , Andrew Sarris 1662 Words | 5 Pages. The Auteur Theory: Stanley Kubrick. Zen Buddhism Suzuki! Auteur Theory is great gatsby dream term paper based on database theses and dissertations, three premises, the first being technique, the second being personal style, and the third being . interior meaning. Furthermore, there is no specific order in great gatsby american paper, which these three aspects must be presented or weighted with regard to a film.

An Auteur must give films a distinctive quality thus exerting a personal creative vision and interjecting it into the his or her films. Kubrick made his first film in 1953 and has continued to make films till his death shortly after. 2001: A Space Odyssey , Arthur C. Clarke , Eyes Wide Shut 1210 Words | 4 Pages. ? Auteur “ Auteur n. a film director regarded as having such a significant influence on films that he or she directs as to be . able to rank as their author. (author = originator)” (Pearsall Trumble ed., 1996, p 92) Authorship The idea that 'the author' is the source of meaning and value in artistic texts has been a persistent one. We talk of Shakespeare’s plays or Austen's novels in essays, ways that suggest that William Shakespeare and great american dream term, Jane Austen are uniquely gifted and independent individuals, solely. Owl Purdue Apap! Alfred Hitchcock , Andre Bazin , Auteur theory 735 Words | 3 Pages. The Auteur theory: Stanley Kubrick. Kubrick: Auteur Theory Andrew Sarris said the auteur theory could be broken down into term paper 3 premises and database of african theses, . these premises can be visualized as 3 circles.

The outer circle the middle and the inner. Outer being the director’s technique, the middle being the personal style of the director, and the inner being the gatsby american dream term interior meaning. An auteur can be looked at in a way as someone who reinvents cinema. . Database Theses And Dissertations! An auteur can change the way we think about great gatsby american, cinema. To put a literal definition to the word, an auteur is a filmmaker. Film , Film director , Film theory 1413 Words | 4 Pages. Auteurs Theory and Stanley Kubrick. the auteur theory witch elevates a director as not just a member of the film crew but as the artist bringing his personal style . and personality to a film. Kubrick’s work will be analysed in accordance with the auteur theory in online college application, other to establish that he is one of the best directors in the industry. American Term! The auteur theory makes it possible for a film to be more than a collaborative industrial project but makes it possible for a film to be identified by its director who is seen by owl purdue apap the auteur theory as its. A Clockwork Orange , Arthur C. Clarke , Film 1167 Words | 4 Pages. Great Dream Paper! AUTEUR THEORY “The auteur theory is a way of reading and appraising films through the imprint of an . auteur (author), usually meant to be the director.” Andre Bazin was the founder, in 1951, of Cahiers du cinema and is often seen as the father of auteurism because of his appreciation of the world-view and style of such artists as Charlie Chaplin and Jean Renoir. It was younger critics at the magazine who developed the idea further, drawing attention to ten steps significant directors from the Hollywood.

Alfred Hitchcock , Auteur theory , Cinema of the United States 2660 Words | 8 Pages. CHAPTER 1: Auteur theory in the context of Film theory Although the term auteur dates back to the . 1920s in american dream term, the theoretical writings of essays French film critics and directors of the silent era, it is great gatsby paper worth pointing out that in Germany, as early as 1913, the term ‘author’s film (Autorenfilm) had already been coined (Hayward,2000: 20). Hayward believed that the Autorenfilm emerged partly as a response to d a resume the French Film d’Art (art cinema) movement, which began in 1908. Film d’Art was particularly successful. Term Paper! Alfred Hitchcock , Andre Bazin , Auteur theory 4724 Words | 12 Pages. GARRY MARSHALL- AUTEUR THEORY An auteur is a filmmaker whose movies are characterized by their creative . influence. Garry Marshall is an American filmmaker, he has directed more than 15 films in his career. Garry Marshall’s films The Princess Diaries, Valentines Day and online college application essay help, Overboard share a common theme of love and term, a genre of d a romance and comedy, he likes to use the same actors in his films and have the common plot of a double twist. Garry Marshall likes to keep to great dream term the same character persona and.

Actor , Anne Hathaway , Film 1267 Words | 4 Pages. Auteur Theory : Darren Aronofsky (Black Swan) Auteur Theory : Translated from the essays zen buddhism suzuki French, auteur simply means author. There have been varied perceptions . regarding this theory , its importance and effectiveness. Auteur theory is essentially “a method of gatsby american dream term evaluating films based on the director’s involvement and input”. The concept of research paper apa ‘ Auteur ’ was first introduced by Francois Truffaut in 1954 in A Certain Tendency in French Cinema.(1) In this work he claimed that film is a great medium for expressing the personal ideas of the great gatsby american term paper director. He suggested. Research Paper Proposal Apa! Alfred Hitchcock , Auteur theory , Clint Mansell 1875 Words | 5 Pages. director should be regarded to as an auteur . Auteur Theory refers to great gatsby term the reflection of the online college essay director’s personal . creative vision and style. “ Auteur ” is a French word for “author.” Auteur Theory suggests that a director can use the great gatsby american term commercial equipment of filmmaking in the same way that an suzuki artist uses a paintbrush to paint. Great Gatsby American Dream Paper! Auteur Theory is an avenue for the personal artistic expression of the director. The film theoretician, Andre Bazin , explained that, “ Auteur theory is database of african and dissertations a way of paper choosing the personal.

Art , Auteur theory , Citizen Kane 399 Words | 3 Pages. ?Why not ANDRES BONIFACIO as our national hero? The Philippines also known as “The Pearl of the Orient” like all other great countries had . difficult beginnings. Andres Bonifacio and Jose Rizal two of the proponents that instigated our great struggle for freedom, they who reminded us of the owl purdue apap pride and strength of a Filipino, they who showed us that we are people who will never give up and will fight no matter what. Great Paper! One became the online application voice of the people; leading the propaganda movement that inspired the. Andres Bonifacio , Emilio Aguinaldo , Jose Rizal 1563 Words | 6 Pages. A Brief Account on Andre Lefevere’s Manipulation Theory. A Brief Account on Andre Lefevere’s Manipulation Theory Abstract: In 1990s,there was a wave breakthrough in translation . theory made by Andre Lefevere and Susan Bassnett.

They went beyond the word-to-word or text level in translation studies and developed it to culture studies, later termed by american dream Mary Snell-Hornby as ‘cultural turn’. This article mainly deals with one kind of essay writing culture turn put forward by Andre Lefevere, that is, translation as rewriting. American Dream Term! ????? . Fiction , Literary criticism , Literary theory 1936 Words | 6 Pages. can the coen brothers be described as auteur. in ten steps writing, several areas, can they truly be described as auteur ? Francois Truffaut was a French critic, who wrote for Cahiers du Cinema, and his most . notable article “Une Certain Tendance du Cinema Francais,”, written in american term, 1954, introduced the writing idea of the great gatsby american dream auteur director. Truffaut argued that “An auteur is primarily and suzuki, exclusively a director. Gatsby American Term Paper! Mise-en-scene is the auteur stripped bare; . and how to resume, this alone defines the gatsby american term paper auteur ”. Truffaut believed that an d a resume auteur director injected his own personal creativity into. Academy Award for Best Picture , Blood Simple , Burn After Reading 1858 Words | 3 Pages. An Argument Against Auteur Theory. Great American Dream Paper! Greatness.

The term Auteur seems to bless a privileged group of owl purdue apap filmmakers with an almost messiah-like legacy. Men such as Alfred Hitchcock, . John Ford and Fritz Lange are believed to inhabit the ranks of the cinematic elite, and not surprisingly most critics are more than willing to bestow upon them the title of Auteur . By regarding filmmaking as yet another form of art, Auteur theory stipulates that a film is the direct result of its director's genius. With the emerging prominence of auteur based criticism. Academy Award for great dream Best Picture , Alfred Hitchcock , Andrew Sarris 2739 Words | 8 Pages. The Place of an Auteur Director in the Nigerian Video Film Industry. the film is the creative intelligence who shapes the entire film in a manner parallel to how we think of literary works being authored. In his essay, . ‘Notes on The Auteur Theory in 1962,’ Andrew Sarris, one of the key proponents of Auteur theory corroborates the owl purdue apap above position as he posits that one of the premises of the great gatsby american paper Auteur theory is the distinguishable personality of the director as a criterion of value. He argues: Over a group of films, a director must exhibit certain recurrent characteristics. Andre Bazin , Auteur theory , Film 3689 Words | 11 Pages.

Use a range of auteur theories to examine the work of two significant directors you have studied on this module. One director . Database And Dissertations! should have produced the majority of their work prior to 1960 and the other should have produced it from the 1970s onwards. Discuss the great american dream origins and main developments of auteur theory then examine the works of Howard Hawks and Martin Scorsese with relevance to their status as auteur directors. In having their films examined as auteurs of the cinema, both Howard Hawks. Andre Bazin , Andrew Sarris , Auteur theory 1744 Words | 7 Pages. editing of the ten steps essay film, as well as managing the script into gatsby american dream paper a sequence of database of african and dissertations shots, coordinating the actors in the film and supervising musical aspects. The . Auteur Theory suggests that films contain certain characteristics or ‘signatures’ that reflect the director’s individual style and give a film its personal and unique stamp.

Hayao Miyazaki is one such auteur whose entertaining plots, compelling characters and dream, breathtaking animation in his films have earned him international renown from critics as well as. Anime , Film director , Hayao Miyazaki 1129 Words | 3 Pages. to describe the d a resume mark of a film director on his films. A director can be considered an auteur if about five of his film depict a certain style . that is definitely his own. In other words, much like one can look at a painting and tell if it is a Monet, a Renoir, or a Degas, if a film director is an auteur , one can look at his film and tell by style and recurring themes that it was made by a certain director. In auteur films, the director is many times what brings an audience to the theater, instead of. Alfred Hitchcock , Aparajito , Auteur theory 1333 Words | 4 Pages. role in adult personality.

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The Importance of Authorship in Film Studies. this essay, I will talk about the auteur theory and the directors who I think can be considered this status. Writing! The French term . ‘ auteur ’ means author and it came about by French film critics in the 1920s. Great Gatsby Dream Paper! However much controversy regarding the use of online application essay auteur was brought about by great gatsby dream term paper a new film critic group called Cahier Du Cinema. It was founded by a French critic called Francois Truffaut. film club and owl purdue apap, met Andre Bazin , a French critic, who becomes his protector.

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International relations , Nuclear weapon , Regime theory 1449 Words | 4 Pages. ?Welcome all, to an evaluation on the auteur of British director Guy Ritchie, as seen in two of his most successful films. Ritchie was born on essays suzuki, . the 10th September 1968 in Hatfield, England.. Ritchie’s directing career started in 1995, where he directed a 20 minute short film entitled The Hard Case. Three years after that, Ritchie directed his second film, and his first ever feature film, Lock, Stock, and Two Smoking Barrels ( commonly referred to as Lock, Stock. Then, not two years later, Ritchie. Gatsby American Term! Benicio del Toro , Film , Gangster films 1158 Words | 3 Pages. Ten Steps Essay Writing! ? A Comparison of Theories Andre Carrington MFCC/556 February 18, 2014 Donna Clark A Comparison of american . Owl Purdue Apap! Theories Introduction There are an abundance of valid theoretical orientations to choose from in family therapy. For a family therapist to be effective, it is important for them to great dream choose a theory they feel comfortable using.

This selection process must involve previewing the suzuki different theories to see which ones match their own unique personal style of dream counseling. This paper will. Dysfunctional family , Family , Family therapy 1560 Words | 5 Pages. An auteur is a director who personal creative vision and style is expressed through films. The term auteur is originated in . France and is French for ten steps essay author. There are different ways in which a director can express their vision in films and great american term paper, show who they are. There are many directors that are considered to be a auteur such as: Quentin Tarintino, Tim Burton, Kathryn Bigelow, Stanley Kubrick and Woody Allen. The director I have chosen as an auteur is Spike Lee. Lee’s Life: Spike Lee is an American.

Do the Right Thing , Feature film , Film 1064 Words | 3 Pages. ? Theory X and research paper apa, Theory Y From Wikipedia, the free encyclopedia This article may require copy editing for grammar, style, cohesion, . tone, or spelling. You can assist by editing it. (October 2014) Theory X and Theory Y are theories of human motivation, created and developed by Douglas McGregor at the MIT Sloan School of Management in the 1960s, that have been used in human resource management, organizational behavior, organizational communication and organizational development. They describe two contrasting. Abraham Maslow , Douglas McGregor , Management 1113 Words | 3 Pages. Theory X, Theory Y by Douglas McGregor is a motivation theory . Douglas McGregor is american paper a social psychologist and . applied two sets of assumptions to the organizational structure called Theory X and research paper proposal apa, Theory Y. His theory is based on managerial views of human beings. In his book, The Human Side of Enterprise, he outlined a new role for managers. He stated that managers should assist subordinates in gatsby american term, reaching their full potential, rather than commanding and controlling. Theory X is negative and college application, Theory Y can. Behavior , Douglas McGregor , Goal 1381 Words | 4 Pages. LYNCH'S GENESIS AS A HEROIC AUTEUR.

Lynch's decision to paper stay in ten steps, the Process but to rule in small personal films rather than to serve in large corporate ones. Whether you believe he's a good . auteur or a bad one, his career makes it clear that he is indeed, in the literal Cahiers du Cinema sense, an auteur , willing to make the sorts of sacrifices for great gatsby american paper creative control that real auteurs have to make-choices that indicate either raging egotism or passionate dedication or a childlike desire to run the essay sandbox, or all three. Blue Velvet , David Lynch , Dino De Laurentiis 1052 Words | 3 Pages. ? Theory X and Theory Y Douglas McGregor (1906 - 1964) is one of the american term forefathers of management theory and one of . the top business thinkers of all time. Online College Help! He was a social psychologist who became the President of Antioch College. He later became a professor of management at Massachusetts Institute of great gatsby american Technology (he was succeeded by suzuki Warren Bennis).

His book The Human Side of Enterprise (1960) had a profound influence on the management field, largely due to great gatsby american dream paper his Theory X and Theory Y. McGregor developed. Control , Douglas McGregor , Goal 1209 Words | 4 Pages. Theory X and paper, Theory Y Understanding Team Member Motivation What motivates employees to go to work each morning? Many people . get great satisfaction from their work and take great pride in it; Others may view it as a burden, and simply work to survive. Great Gatsby American Term Paper! This question of motivation has been studied by management theorists and social psychologists for decades, in attempts to identify successful approaches to paper proposal apa management. Social psychologist Douglas McGregor of MIT expounded two contrasting theories. Control , Douglas McGregor , Knowledge management 915 Words | 3 Pages. 1.0 THEORY X1.1 THEORY X ASSUMPTIONS | 2-3 | 2 | 2.0 THEORY Y2.1 THEORY Y ASSUMPTIONS | 4-5 | . Great Gatsby American Dream Paper! 3 | 3.0 BIG FIVE PERSONALITY TRAITS-OPENNESS-EXTRAVERSION-NEUROTICISM-CAREFULNESS-SOCIABILITY | 6-11 | 1.0 THEORY X What is theory X? The “ Theory X” management theory holds that the average employee has little ambition, dislikes work and must be coerced, controlled and directed to achieve organizational objectives.

Those in management who believe the behavioral assumption of “ Theory X” take. Big Five personality traits , Management , Neuroticism 1711 Words | 7 Pages.

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Use of stolen credit card and/or any credit card fraud is considered to be a serious crime. closely cooperates with our payment provider to online help prevent and fight online fraud. In case of any online fraud, appropriate state authorities will be contacted immediately. By doing a chargeback, you agree to gatsby american give up all your rights to the Paper automatically. At the same time, you authorize to publish the completed Paper and start the authorship procedure that will allow us to determine if you have used any parts of the Paper. Essay? The procedure may include contacting your school officials and/or posting your full details along with the completed Paper online. reserves the right to change its prices at any time in its sole discretion and such changes or modifications shall be posted online at gatsby dream the Website and become effective immediately without need for further notice to owl purdue apap any Client and/or user. We care about our Clients and are always looking for ways to great american dream term paper offer them the best value for money. One method we use is a discount system., at its sole discretion, shall have the right to provide our Clients with discount programs as described more fully and published on the Website.

According to online college our loyalty program, you earn back 10% of your total bill in american dream term paper Points (1 currency unit (inter alia USD/ EUR/ GBP etc.) = 1 Point) after you make your first order. Your Points are accumulated on your Credit Balance. “Credit Balance” is an proposal apa account for Points of a Client which can be used for future purchases on the Website exclusively. You can use your Points for your next purchases on the Website exclusively. Your Points cannot be refunded. The discount may be obtained by the use of the promo code. Great American Dream Paper? The amount of Points added to the Credit Balance is calculated on the basis of the order price excluding the applied discount (if any). Later, 5% of every next order (not including credits) is added to your Credit Balance. will issue a refund to ten steps essay you only according to dream paper these Terms. offers a 14-day money back period for Papers less than 20 pages and a 30-day period for Papers more than 20 pages (”Refund Period”). Refund Period begins on essay the date of Client`s order deadline and gatsby term, expires on the last day of the Refund Period. In case you are not satisfied with any of the Services, you can submit a refund request according to these Terms within the d a resume Refund Period. Once the american dream Refund Period elapses, will not refund any amounts paid. If the order is how to d a, not completed and/or the Paper is not downloaded or delivered in gatsby american term paper its complete form by or to you, the full refund is issued at any time. In the owl purdue apap event of order cancellation, the funds will be debited back only to the account of the initial payment within 5-7 business days from the great gatsby american dream term time of cancellation request. In other case assesses refund requests on research paper a case-by-case basis as there are usually unique reasons as to why a refund request is made. Please note that if you request a refund, we may require documented proof that the gatsby dream term quality of your order is low (e.g., scan copy of your instructor’s feedback, plagiarism report, etc.). Should you feel it necessary to make a refund request, we will immediately forward your order to our Quality Assurance Department.

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All revisions must be based on the original order instructions. If at the time of the revision request you provide new, additional, or differing instructions, this will be interpreted as an great gatsby dream application for new Paper and thus, will require an additional payment. Suzuki? Furthermore, should you request a revision after the Revision Period, it will also be considered as a new order requiring an additional payment. We may require you to great gatsby american term paper supply us with personal identifying information, and we may also legally consult other sources to obtain information about you. By accepting these Terms and Conditions, you authorize us to make any inquiries we consider necessary to validate the application information that you provide us with. We may do this directly or by great american dream term paper, verifying your information against third party databases; or through other sources.

Essentially, verification procedure involves, inter alia, confirming that the ten steps order is authentic and that the cardholder is aware of charges by placing a phone call to them, and in certain cases by great gatsby american dream term, requesting some additional documents to research paper proposal be submitted for verification to our Risk Department. In order to gatsby american dream term paper ensure timely delivery of your order, this procedure must be completed quickly and without delay. Therefore, it is application, vital to provide accurate and valid phone numbers. Failure to verify an order may result in order cancellation or the order being placed on hold. You consent to our processing your personal information for the purposes of great gatsby, providing the ten steps writing Services, including for verification purposes as set out gatsby american herein. You also consent to the use of such data for communicating with you, for essay help statutory and accounting purposes. You acknowledge that you have read and consented to's Privacy Policy. LIMITATIONS OF LIABILITY. will not be liable to you in great term relation to owl purdue apap the contents of, the gatsby use of, or otherwise in connection with, this Website: for failure to essays zen buddhism learn the material covered by the Paper; and. for your final grade; and.

for the outcome or consequences of great gatsby term paper, submission the Paper to college application essay any academic institution; and. excludes all liability for damages arising out of or in great dream term paper connection with your use of this Website. The latter includes, without limitation, damage caused to your computer, computer software, systems and programs and owl purdue apap, the data thereon, or any other direct or indirect, consequential and great gatsby american term paper, incidental damages. The Paper provided to you by remains our property and database theses and dissertations, is the great dream paper subject to copyright and other intellectual property rights under local and international laws conventions. The Paper is intended for your personal use only and it may not be used, copied, reproduced, distributed, transmitted, broadcast, displayed, sold, licensed, or otherwise exploited for any other purposes without our prior written consent. You agree not to engage in the use, copying, or distribution of Papers other than expressly permitted herein. We post Clients` testimonials on our Website which may contain personal information (first name or initials). Hereby by accessing or using this Website, you provide us with your consent to post your first name/initials along with your testimonial on our Website. We ensure our posting these testimonials does not interfere with your confidentiality.

If you wish to request the removal of your testimonial, you may contact us at [emailprotected] NOTIFICATION OF CHANGES. reserves the right to change these Terms and Conditions at any time and your continued use of the ten steps essay Website will signify your acceptance of paper, any adjustment, improvements and/or alterations to these Terms and Conditions. Zen Buddhism? You are, therefore, advised to re-read these Terms and Conditions on a regular basis. This web site is owned and operated by Viatta Business Ltd. HEXO+ Self-Flying Camera Drone, with a suggested retail price of $1,249.00 USD («Main prize»). FreePage (single use) SMS inform (single use) Plagiarism Report (single use) 50$ to your bonus balance which you can use in great gatsby american dream term paper 365 days 100$ to your bonus balance which you can use in 365 days. 2. Promotional Period.

The promotion begins on 7.18.2017, at 9:00 am and essay, ends on 7.28.2017 at 10:00 pm. This Privacy Policy (“Policy”) describes how information about You is collected, used and disclosed and great dream term, provides other important privacy information, describes when and how we may change this Policy, and tells You how to contact us with any questions or comments. We collect information about You and computer(s) You use when You use our Services or otherwise interact with us. “Personal Information” means information that we directly associate with a specific person or entity (for example: name; addresses; telephone numbers; email address; payment information; device location etc.). “Client”, “User”, “You” and owl purdue apap, “Your” refers to you, the person accessing this Website and accepting these Privacy Policy. Any use of the above terminology or other words in the singular, plural, capitalization and/or he/she or they, are taken as interchangeable and therefore as referring to same. HOW INFORMATION ABOUT YOU IS COLLECTED. We collect information about You in three primary ways: Information You Provide.

We collect information that You provide to great gatsby american term us when You apply for and use and/or purchase our Services or otherwise communicate with us. For example, some of the ways You may provide information to us include: When You purchase our Services, the essay payment system will require your personal, contact, billing and gatsby american dream term, credit information. When You establish or modify Your user account online, We may collect user identification information, passwords, and/or security question responses that You will use for future sign-on. When You interact with our Customer Service representatives, enter information on our Website, submit survey responses, or pay for Services, we may also collect Personal Information and other information. D A? We may monitor and record phone calls, e-mails, live chats, or other communications between You and our Customer Service representatives or other employees or representatives. Information We Collect Automatically. We automatically collect a variety of information associated with Your use of great american dream term paper, our Services. Each time You visit the Website, Personal Information is automatically gathered. In general, this information does not identify You personally. Examples of automatically collected personal information include, but are not limited to: IP address, Collection Date, Publisher Name, Connection Speed, Day of of african theses and dissertations, Week Time of Day (hour), Language settings, Country, City (relating to gatsby american dream term IP address, if available).

For example, some of the ways we may automatically collect information include: Cookies and similar technologies. A “cookie” is a small text file that a web site can place on zen buddhism suzuki Your computer's hard drive in gatsby american term paper order, for example, to collect information about Your activities on how to the Website. The cookie transmits this information back to the Website's computer, which, generally speaking, is the only computer that can read it. We need to use cookies on the Website to enhance the user experience and avoid multiple logins or password authentication requests. We may use, or we may engage third-parties to use on our behalf, cookies or similar web tags (small data text files placed on your computer or device) or similar technologies to identify Your computer or device and record Your preferences and gatsby american term, other data so that our Website can personalize Your visit(s), see which areas and features of essay, our Website are popular, and improve our Website and Your experience. Depending upon Your computer, You may be able to set Your browser(s) to reject cookies or delete cookies, but that may result in the loss of some functionality on american paper the Website. We may also use web beacons (small graphic images on database theses a web page or an HTML e-mail) to monitor interaction with our websites or e-mails. Web beacons are generally invisible because they are very small (only 1-by-1 pixel) and great term, the same color as the background of the web page or e-mail message. Web Browsing Activity.

When accessing our Website, We automatically collect certain information about Your computer and Your visit, such as your IP address, browser type, date and proposal apa, time, the gatsby american dream term paper web page You visited before visiting our Website, Your activities and purchases on our Website, and other analytical information associated with the Website. Information From Other Sources. We may also obtain information about You from how to d a resume, other sources. For example, We may receive credit information from dream, third-party sources before initiating Your service. We may also purchase or obtain Personal Information (for example, e-mail lists, postal mail lists, demographic and marketing data) from others.

HOW WE USE INFORMATION WE COLLECT ABOUT YOU. We use the essays information We collect for a variety of gatsby dream paper, business purposes, such as: To provide and bill for Services You purchase; To deliver and confirm Services You obtain from us; To verify Your identity and maintain a record of d a, Your transactions and interactions with us; To provide customer services to You; To create, modify, improve, enhance, remove or fix our Services and their performance;

To identify and great paper, suggest products or services that might interest You; To make internal business decisions about current and future Service offerings; To provide You customized user experiences, including personalized Services offerings; To protect our rights, interests, safety and owl purdue apap, property and great gatsby dream, that of d a resume, our customers, service providers and other third parties; and. To comply with law or as required for legal purposes. We may use Personal Information for investigations or prevention of fraud or network abuse. We may use information we collect to contact You about great gatsby dream term paper, our and/or third-party products, services, and offers that We believe You may find of interest. We may contact You by telephone, postal mail, e-mail, or other methods. You may see advertisements when You visit our Website.

We may help advertisers better reach our customers by providing certain customer information, including geographic information, language preferences or demographic information obtained from other companies. This information is used by advertisers to determine which ads may be more relevant to suzuki You. However, we do not share Personal Information outside of our corporate family for advertising purposes without Your consent. WHEN WE SHARE INFORMATION COLLECTED ABOUT YOU. We do not sell, license, rent, or otherwise provide Your Personal Information to unaffiliated third-parties (parties outside our corporate family) without Your consent. We may, however, disclose Your information to unaffiliated third-parties as follows:

With Your Consent. We may disclose Personal Information about You to gatsby term paper third-parties with Your consent. We may obtain Your consent in writing; online, through “click-through” agreements; when You accept the d a resume terms of disclosures for certain Services; orally, when You interact with our customer service representatives. We encourage You not to share Your password. If You provide Your user account password and/or security question responses to third parties they will have access to Your Personal Information when they access Your user account with Your account password. To Our Service Providers. We may disclose information to third-party vendors and partners who complete transactions or perform services on american dream term paper our behalf (for example, credit/debit card processing, billing, customer service, auditing, and database theses, marketing). In a Business Transfer. We may sell, disclose, or transfer information about great gatsby dream, You as part of owl purdue apap, a corporate business transaction, such as a merger or acquisition, joint venture, corporate reorganization, financing, or sale of company assets, or in term the unlikely event of paper apa, insolvency, bankruptcy, or receivership, in dream term which such information could be transferred to third-parties as a business asset in the transaction.

For Legal Process Protection. We may disclose Personal Information, and other information about You, or Your communications, where we have a good faith belief that access, use, preservation or disclosure of such information is reasonably necessary: to satisfy any applicable law, regulation, legal process or enforceable governmental request; to enforce or apply agreements, or initiate, render, bill, and collect for services and products (including to collection agencies in paper proposal order to obtain payment for our products and gatsby dream term, services); to protect our rights or interests, or property or safety or that of others; in connection with claims, disputes, or litigation – in court or elsewhere; to facilitate or verify the owl purdue apap appropriate calculation of taxes, fees, or other obligations; or. in an emergency situation. We may provide information that does not identify You personally to third-parties for marketing, advertising or other purposes. HOW WE STORE AND PROTECT THE INFORMATION COLLECTED ABOUT YOU. Protecting Your Information. We use a variety of great, physical, electronic, and procedural safeguards to protect Personal Information from unauthorized access, use, or disclosure while it is writing, under our control. Unfortunately, no data transmission over the internet can be guaranteed to be completely secure. As a result, although we will utilize such measures, we do not guarantee You against the loss, misuse, or alteration of Personal Information under our control, and You provide Personal Information to us at Your own risk. You should always take care with how You handle and great gatsby american dream term paper, disclose your Personal Information and should avoid sending Personal Information through insecure e-mail, social networks or other internet channels. Retention and Disposal.

We retain information only for as long as we have a business or tax need or as applicable laws, regulations and/or government orders allow. When we dispose of database of african, Personal Information, we use reasonable procedures designed to erase or render it unreadable (for example, shredding documents and gatsby american dream term, wiping electronic media). PRIVACY POLICY UPDATES. How We Communicate Changes to This Policy. We may update this Policy at any time to provide updates to or clarification of our practices. If we make changes we may provide You with additional notice (such as adding a statement to owl purdue apap the homepage of our Website or sending You a notification).

You should refer to gatsby american dream this Policy often for the latest information and the effective date of any changes. This web site is owned and operated by Viatta Business Ltd . A Partner is an d a resume individual who refers customers. Gatsby American Term? A Referral is an individual who requests a service via the referral link given by a Partner. With the how to d a resume first order, a Referral acquires a 15% discount on the order, while a Partner receives $50 to the Referral Balance. With further purchases, a Partner earns 5% of the Referral’s total order price. All money earned with the Referral Program is stored on great dream term paper your Referral Balance.

A Partner can transfer the college application help money to great gatsby term the Bonus Balance and use it to purchase a service. It is possible to transfer the sum to the Partner’s PayPal account (no less than $20).